Kärkkäinen S
J Chromatogr. 1984 Dec 12;336(2):313-9.
A sensitive, selective and reproducible reversed-phase high-performance liquid chromatographic method is described for the quantification of sotalol in human serum and urine. Sotalol and the internal standard, atenolol, were extracted from alkalinized serum and urine (pH 9.0) into 1-butanol--chloroform (20:60, v/v). The organic phase was evaporated, and to the residue was added 0.1 M sulphuric acid (serum analysis) or mobile phase (urine analysis). The mobile phase consisted of 0.01 M phosphate buffer (pH 3.2) and acetonitrile (20:80, v/v) containing 3 mM n-octylsodium sulphate. The flow-rate was 1.5 ml/min. The retention times of atenolol and sotalol were 7 and 10 min, respectively. Ultraviolet detection at 226 nm made it possible to achieve a detection limit of 0.03 mumol/l.
本文描述了一种灵敏、选择性好且可重现的反相高效液相色谱法，用于定量测定人血清和尿液中的索他洛尔。索他洛尔和内标阿替洛尔从碱化的血清和尿液（pH 9.0）中萃取至1-丁醇-氯仿（20:60，v/v）中。有机相蒸发后，向残渣中加入0.1 M硫酸（用于血清分析）或流动相（用于尿液分析）。流动相由0.01 M磷酸盐缓冲液（pH 3.2）和含有3 mM正辛基硫酸钠的乙腈（20:80，v/v）组成。流速为1.5 ml/min。阿替洛尔和索他洛尔的保留时间分别为7分钟和10分钟。在226 nm处进行紫外检测，使得检测限可达0.03 μmol/l。
