Moseley M E, Nishimura M C, Pitts L H, Bartkowski H M, James T L
Magn Reson Imaging. 1984;2(3):205-9. doi: 10.1016/0730-725x(84)90006-7.
Proton nuclear magnetic resonance relaxation times, T1 and T2, of water in unfixed gray and white matter from normal and edematous rabbit brain tissues were measured in vitro at 23 degrees C and 100 MHz to evaluate the effects of the temperature (-25 degrees C to 37 degrees C) and duration (0 to 96 h) of tissue storage on relaxation times. T1 and T2 tended to decrease during storage, probably from slow dehydration of the tissue. This effect was greatest in tissues stored at 37 degrees C and least in those stored at 4 and -25 degrees C; decreases in T1 and T2 were greater in white matter than in gray matter. Freezing brain tissue to -25 degrees C caused a sudden decrease in the T2 of normal white matter. Relaxation times were constant for 5 h in tissues stored at 23 degrees C and for 40 h at 4 degrees C. These results correlated well with corresponding tissue water loss.
在23摄氏度和100兆赫的条件下，对来自正常和水肿兔脑组织的未固定灰质和白质中的水的质子核磁共振弛豫时间T1和T2进行了体外测量，以评估组织储存温度（-25摄氏度至37摄氏度）和持续时间（0至96小时）对弛豫时间的影响。在储存过程中，T1和T2往往会降低，这可能是由于组织缓慢脱水所致。这种影响在37摄氏度储存的组织中最大，在4摄氏度和-25摄氏度储存的组织中最小；白质中T1和T2的降低幅度大于灰质。将脑组织冷冻至-25摄氏度会导致正常白质的T2突然下降。在23摄氏度储存的组织中，弛豫时间在5小时内保持恒定，在4摄氏度储存的组织中，弛豫时间在40小时内保持恒定。这些结果与相应的组织水分流失密切相关。
