van Voorthuizen H, Kluft C
Thromb Haemost. 1984 Dec 29;52(3):350-3.
Assays for antithrombin III activity using the chromogenic substrate S-2238 were automated in various modifications on various instruments. Standard curves for the assay showed a deviating "0% point" in several but not all modifications. Addition of polyethylene glycol 6000/8000 (0.40-1.0%) minimizes this deviation. However, the deviation of the 0% point was not completely abolished in every modification of the test. Examination of Lineweaver-Burk plots showed nonlinearity at high concentrations of S-2238 as used in the antithrombin III assays. Addition of Tween-80 (0.01%) restores the Lineweaver-Burk plot and, at the same time, the standard curve in the assays is found to be linear. Therefore, we recommend the use of PEG 6000/8000 as well as Tween-80 in automated assay systems.
使用发色底物S-2238检测抗凝血酶III活性的方法在各种仪器上进行了多种改进并实现了自动化。该检测的标准曲线在几种（但并非所有）改进方法中显示出“0%点”偏差。添加聚乙二醇6000/8000（0.40 - 1.0%）可使这种偏差最小化。然而，在每次检测改进中，0%点的偏差并未完全消除。对Lineweaver - Burk图的检查表明，在抗凝血酶III检测中使用的高浓度S-2238下存在非线性。添加吐温-80（0.01%）可恢复Lineweaver - Burk图，同时发现检测中的标准曲线呈线性。因此，我们建议在自动化检测系统中同时使用聚乙二醇6000/8000和吐温-80。
