Gram J, Jespersen J
Clin Chem. 1985 Oct;31(10):1683-8.
When manual assays of antithrombin-III with use of different synthetic chromogenic peptide substrates are modified for a centrifugal analyzer, the downward deflection of the reference curve at the zero point raises a serious problem, because the position of the zero value is included in the calculation of the reference curve. Complete linearity was obtained by addition of polyethylene glycol 6000 to the thrombin solution and Tween 80 to the substrate solution in final concentrations of 1 g/L and 100 mg/L, respectively. The additives increased the concentration of active thrombin in the solutions by preventing its adsorption and aggregation, and the solubility of the substrates in aqueous media is increased. The precision of the optimized method, tested in routine assays during three months, yielded an estimated day-to-day CV of 3.4%.
当使用不同的合成生色肽底物对手动抗凝血酶 III 检测方法进行改进以用于离心分析仪时，参考曲线在零点的向下偏移会引发严重问题，因为零点值的位置包含在参考曲线的计算中。通过分别向凝血酶溶液中添加终浓度为 1 g/L 的聚乙二醇 6000 和向底物溶液中添加终浓度为 100 mg/L 的吐温 80，可实现完全线性。这些添加剂通过防止活性凝血酶的吸附和聚集，提高了溶液中活性凝血酶的浓度，同时也增加了底物在水性介质中的溶解度。在三个月的常规检测中对优化方法的精密度进行测试，估计的日间变异系数为 3.4%。
