Kolde H J
Clin Chem. 1986 Aug;32(8):1554-6.
To investigate whether the characteristics of a commercial test kit for antithrombin III (Berichrom Antithrombin III) could be influenced by surfactants such as Tween-80 or polyethylene glycol (PEG), we performed some experiments with the original kit reagents and with the reagents dissolved in surfactants. Neither the reliability of the calibration curve nor the data for precision and assay kinetics were amended by the addition of either PEG to the (human) thrombin reagent or Tween-80 to the chromogenic substrate. In the same test system, assays with some other chromogenic substrates and with bovine thrombin showed comparable behavior. Evidently, if one follows the working scheme proposed for this kit, the use of surfactants is not warranted.
为了研究抗凝血酶III商业检测试剂盒（Berichrom抗凝血酶III）的特性是否会受到吐温80或聚乙二醇（PEG）等表面活性剂的影响，我们使用原始试剂盒试剂以及溶解在表面活性剂中的试剂进行了一些实验。向（人）凝血酶试剂中添加PEG或向显色底物中添加吐温80，均未改变校准曲线的可靠性以及精密度和测定动力学数据。在同一检测系统中，使用其他一些显色底物和牛凝血酶进行的检测显示出类似的结果。显然，如果按照该试剂盒建议的工作方案进行操作，则无需使用表面活性剂。
