Elsayed S, Apold J, Aas K, Bennich H
Int Arch Allergy Appl Immunol. 1976;52(1-4):59-63. doi: 10.1159/000231668.
In attempts to locate the allergenic active sites of fragment TM 1 of allergen M from cod, the allergenicity of the tryptic hydrolysis peptides was examined both in vivo and in vitro. A duodecapeptide (residues No. 33-44) was tentatively suggested to incorporate at least one allergenic determinant. All the tryptic peptides were less active than allergen M as shown by the in vivo titration experiments. The activity of the peptides was not due to contamination of intact allergens.
为了确定鳕鱼过敏原M片段TM 1的致敏活性位点，对胰蛋白酶水解肽的致敏性进行了体内和体外检测。初步推测一个十二肽（第33 - 44位氨基酸残基）至少包含一个致敏决定簇。如体内滴定实验所示，所有胰蛋白酶肽的活性均低于过敏原M。这些肽的活性并非源于完整过敏原的污染。
