Elsayed S, Apold J
Int Arch Allergy Appl Immunol. 1977;54(2):171-5. doi: 10.1159/000231821.
The peptides P1B3 (amino acid residues No. 88-113) and LT3N1 (residues No. 88-108), obtained from the limited tryptic hydrolysis of fragment TM 2 from codfish allergen M inhibited the interaction between allergen M and reaginic IgE antibodies both in vitro (87 and 62% RAST inhibition, respectively) and in vivo (total PK test inhibition). The peptide A7B1 (residues No. 97-113) overlapping the former at the COOH-terminus, was inactive in PK test inhibition experiments and produced only a minor inhibition of RAST (15%). The findings suggest that the nonapeptide residue No. 88-96 is partially indispensible for the allergenic activity of fragment TM 2, and is likely to incorporate allergenic determinant(s).
从鳕鱼过敏原M的片段TM 2经胰蛋白酶有限水解得到的肽P1B3（氨基酸残基第88 - 113号）和LT3N1（残基第88 - 108号），在体外（分别有87%和62%的放射性过敏原吸附试验抑制率）和体内（完全被动皮肤过敏试验抑制）均抑制了过敏原M与反应素IgE抗体之间的相互作用。在COOH末端与前者重叠的肽A7B1（残基第97 - 113号），在被动皮肤过敏试验抑制实验中无活性，且仅对放射性过敏原吸附试验产生轻微抑制（15%）。这些发现表明，第88 - 96号九肽残基对于片段TM 2的致敏活性部分不可或缺，并且可能包含过敏决定簇。
