Jacobs M M, Burnett C M, Penicnak A J, Herrera J A, Morris W E, Shubik P, Apaja M, Granroth G
Drug Chem Toxicol. 1984;7(6):573-86. doi: 10.3109/01480548409042820.
The chronic toxicologic and carcinogenic potential of two oxidative and twelve non-oxidative hair dyes has been evaluated. The dyes were skin painted up to 3 times weekly on groups of 60 male and 60 female Eppley Swiss mice. Treatments were carried out for 20 months followed by terminal sacrifice. Nine months after treatments were initiated an intermediate sacrifice of ten mice per sex per group was carried out. Body weights and survival differed little between appropriate male and female treatment and control groups. Differences between treated and control groups in absolute and relative liver and kidney weights and in hematological and urinary values were not considered to be indicative of toxicologic effects. Microscopic examinations of the skin revealed occasional hyperplasia, necrosis, ulceration and other lesions not significantly increased by dye treatment. Chronic inflammation of the skin was observed in the control and treated mice and was significantly increased by one non-oxidative dye. The predominant tumors diagnosed were liver hemangioma, lung adenoma and malignant lymphoma. There was a statistically significant increase in the incidence of malignant lymphoma in female mice in 3 treated groups when compared to control group 2, but the differences were not significant when these groups were compared to control group 1. In addition the values in these 3 groups were within the range of control values for this tumor in female mice in the Eppley colony. No other tumors occurred at significantly increased frequencies in treated mice. We conclude that toxicological and carcinogenic effects were not clearly induced by the hair dye formulations.
已对两种氧化型和十二种非氧化型染发剂的慢性毒理学和致癌潜力进行了评估。每周对60只雄性和60只雌性埃普利瑞士小鼠分组进行多达3次的皮肤涂抹处理。处理持续20个月，之后进行终末处死。在开始处理9个月后，每组每种性别处死10只小鼠进行中期处死。在相应的雄性和雌性处理组与对照组之间，体重和存活率差异不大。处理组与对照组在肝脏和肾脏的绝对重量和相对重量以及血液学和尿液值方面的差异，不被认为表明存在毒理学效应。皮肤的显微镜检查显示偶尔有增生、坏死、溃疡和其他病变，染料处理并未使其显著增加。在对照组和处理组小鼠中均观察到皮肤慢性炎症，一种非氧化型染料使其显著增加。诊断出的主要肿瘤为肝血管瘤、肺腺瘤和恶性淋巴瘤。与对照组2相比，3个处理组的雌性小鼠恶性淋巴瘤发病率有统计学显著增加，但与对照组1相比，这些组之间的差异不显著。此外，这3组的值在埃普利种群雌性小鼠该肿瘤的对照值范围内。在处理组小鼠中，没有其他肿瘤的发生频率显著增加。我们得出结论，染发剂配方未明确诱导毒理学和致癌效应。
