Gould K G, Young L G, Hinton B T
Arch Androl. 1984;12 Suppl:9-17.
To further investigate changes in the sperm surface occurring during epididymal transit and after ejaculation, the surface negative charge on the head of chimpanzee sperm recovered from the caput (n = 4) and cauda epididymis (n = 3) and from the ejaculate (n = 4) was measured. Washed sperm were exposed to colloidal iron at pH 1.6, washed, mounted on carbon plates, and examined in a scanning electron microscope equipped with an energy dispersive X-ray analyzer. This technique permits evaluation of individual sperm in a population and provides information not available when the entire population is measured as a whole. The results demonstrate a net increase in negative charge during epididymal transit and after ejaculation and a change in charge distribution within the population from a unimodal to a tetra modal distribution. Caput sperm showed a unimodal distribution with a mode of 20 +/- 2%. Cauda sperm showed three modes at 23 +/- 1, 34 +/- 3, and 41 +/- 3%. Ejaculated sperm showed four modes at 21 +/- 3, 31 +/- 4, and 40 +/- 2, and 46 +/- 2%. The sperm population in the chimpanzee epididymis and ejaculate is not homogeneous, and this technique will aid in future measurement of fertility of subpopulations of sperm in the ejaculate.
为了进一步研究附睾转运过程中和射精后发生的精子表面变化，对从黑猩猩附睾头（n = 4）、附睾尾（n = 3）以及射精样本（n = 4）中获取的精子头部表面负电荷进行了测量。将洗涤后的精子置于pH值为1.6的胶体铁中，洗涤后，固定在碳板上，并在配备能量色散X射线分析仪的扫描电子显微镜下进行观察。该技术能够对群体中的单个精子进行评估，并提供将整个群体作为一个整体进行测量时无法获得的信息。结果表明，在附睾转运过程中和射精后，负电荷净增加，并且群体内电荷分布从单峰分布变为四峰分布。附睾头精子呈现单峰分布，峰值为20±2%。附睾尾精子呈现三个峰值，分别为23±1%、34±3%和41±3%。射精后的精子呈现四个峰值，分别为21±3%、31±4%、40±2%和46±2%。黑猩猩附睾和射精样本中的精子群体并非同质，这项技术将有助于未来对射精样本中精子亚群的生育能力进行测量。
