Brustad T, Rofstad E K
Institute for Cancer Research, Norwegian Cancer Society, Norwegian Radium Hospital, Montebello, Oslo.
Recent Results Cancer Res. 1988;109:183-97. doi: 10.1007/978-3-642-83263-5_21.
The heat response in vitro and in vivo of five human melanoma xenografts grown in athymic nude mice was studied. The melanomas differed significantly in terms of heat sensitivity both in vitro and in vivo. At least two different mechanisms governed the overall heat response of the melanomas in vivo: the primary cell death, induced during treatment, was due to direct cytotoxic effects of the heat; the secondary cell death, induced after completion of treatment, was due to heat-induced vascular damage. The activation energies for the melanomas were not significantly different in vitro and in vivo at temperatures above the inflection point of the Arrhenius curves. Below the inflection point, on the other hand, the activation energies were higher in vitro than in vivo, probably as a consequence of differences in the physiological conditions in vitro and in vivo. The heat responsiveness of the melanomas in vivo was not related to the radioresponsiveness, whether the heat treatment was given at a low or a high temperature. All melanomas developed thermotolerance after a priming heat treatment. The thermotolerance differed significantly in magnitude among the five melanomas. It was concluded from the thermotolerance data that clinical treatment protocols probably should not prescribe more than one hyperthermic treatment per week.
研究了在无胸腺裸鼠体内生长的五种人黑色素瘤异种移植物在体外和体内的热反应。这些黑色素瘤在体外和体内的热敏感性方面存在显著差异。体内黑色素瘤的总体热反应至少由两种不同机制控制：治疗期间诱导的原发性细胞死亡是由于热的直接细胞毒性作用；治疗完成后诱导的继发性细胞死亡是由于热诱导的血管损伤。在高于阿伦尼乌斯曲线拐点的温度下，黑色素瘤在体外和体内的活化能没有显著差异。另一方面，在拐点以下，体外的活化能高于体内，这可能是由于体外和体内生理条件不同所致。无论热疗是在低温还是高温下进行，黑色素瘤在体内的热反应性与放射反应性均无关。所有黑色素瘤在进行一次预处理热疗后均产生了热耐受性。五种黑色素瘤的热耐受性在程度上有显著差异。从热耐受性数据得出的结论是，临床治疗方案可能不应每周规定超过一次热疗。
