Andrade Z A, dos Reis M G
Mem Inst Oswaldo Cruz. 1984 Jul-Sep;79(3):371-3. doi: 10.1590/s0074-02761984000300012.
Schistosoma mansoni cercariae mechanically transformed into schistosomula were injected, either dead or alive, into the tail vein (2.000 larvae/0,15 ml) of Balb/c mice which were either previously infected with S. mansoni (10 weeks/50 cercariae) or non-infected. Histological examination of the lungs 24, 48, 72 and 96 hours after injection revealed that inflammatory reaction around schistosomula occurred only in the groups injected with dead schistosomula (killed by freezing and thawing). In non-infected animals the reaction was predominantly macrophagic while in those infected many eosinophils appeared around the dead larvae. These results are at variance with those obtained in vitro and suggest that in vivo the participation of eosinophils in the schistosomulum induced reaction in sensitized animals is secondary to the death of the larvae.
将曼氏血吸虫尾蚴机械转化为童虫后，无论是死的还是活的，均经尾静脉注射到Balb/c小鼠体内（2000条幼虫/0.15毫升），这些小鼠要么先前已感染曼氏血吸虫（10周/50条尾蚴），要么未感染。注射后24、48、72和96小时对肺部进行组织学检查发现，仅在注射死童虫（经冻融杀死）的组中，童虫周围出现炎症反应。在未感染的动物中，反应主要为巨噬细胞性，而在感染的动物中，死亡幼虫周围出现许多嗜酸性粒细胞。这些结果与体外实验结果不同，表明在体内，嗜酸性粒细胞参与致敏动物中童虫诱导的反应是幼虫死亡的继发结果。
