Vertongen F, Fondu P, van den Heule B, Cauchie C, Mandelbaum I M
Tumour Biol. 1984;5(6):303-11.
Thymidine kinase (TK) isoenzymes and thymidine phosphorylase (TP) activities have been measured in peripheral mononuclear cells of patients with acute lymphoblastic and monoblastic leukaemia or B-chronic lymphocytic leukaemia, as well as in normal subjects, and also in lymph node cells from patients with non-Hodgkin's lymphoma, with Hodgkin's disease and with benign adenopathies. TK1 isoenzyme activity was highest in acute lymphoblastic leukaemia and in centroblastic lymphoma. Then in progressively decreasing order appeared the Hodgkin's disease values, the centroblastic centrocytic lymphoma values and the benign reactive lymph node cell values. When compared to normal blood mononuclear cells, TP was greatly decreased in acute lymphoblastic leukaemia and slightly but significantly decreased in chronic leukaemia. Monoblastic cells exhibited a unique enzyme pattern; moderately increased TK1 activity and high TP activity. Our results suggest that both enzymes are indicative of the maturation status of leukaemic cells from B lineage. They demonstrate that in lymph node cells, TK1 reflects the proliferative status of both malignant and non-malignant cells and that in monoblastic cells the synthesis of dTMP through de novo synthesis is favoured.
已对急性淋巴细胞白血病、单核细胞白血病或B细胞慢性淋巴细胞白血病患者以及正常受试者外周血单个核细胞中的胸苷激酶（TK）同工酶和胸苷磷酸化酶（TP）活性进行了测定，还对非霍奇金淋巴瘤、霍奇金病和良性腺病患者淋巴结细胞中的这些酶活性进行了测定。TK1同工酶活性在急性淋巴细胞白血病和中心母细胞性淋巴瘤中最高。然后，按逐渐降低的顺序依次为霍奇金病的值、中心母细胞中心细胞性淋巴瘤的值和良性反应性淋巴结细胞的值。与正常血液单个核细胞相比，急性淋巴细胞白血病中TP大幅降低，慢性白血病中TP略有降低但差异显著。单核细胞表现出独特的酶谱；TK1活性适度增加，TP活性较高。我们的结果表明，这两种酶均指示B系白血病细胞的成熟状态。结果表明，在淋巴结细胞中，TK1反映恶性和非恶性细胞的增殖状态，而在单核细胞中，通过从头合成途径合成dTMP的过程更为有利。
