Rakowicz-Szulczyńska E M, Horst A
Arch Immunol Ther Exp (Warsz). 1984;32(6):665-9.
Spleen cells from rats were incubated for 10 min in Eagle's medium containing [125I] IgG or [125I] ovalbumin. Lymphocyte-like and macrophage-like cells were separated by centrifugation in Ficoll-metrizoate solution (d = 1.095). Control spleen cells were incubated in Eagle's medium without [125I] IgG or [125I] ovalbumin and both subpopulations were separated analogically. Lymphocyte-like cells labelled with [125I] IgG or [125I] ovalbumin were incubated for 10 min with control macrophage-like cells and labelled macrophage-like cells with control lymphocyte-like cells. After the incubation time all subpopulations were separated again in Ficoll-metrizoate solution. Radioactivity of [125I] IgG or [125I] ovalbumin was found in cytoplasmic fraction, sap protein fraction (nucleoplasm) and chromatin of control lymphocyte-like and macrophage-like cells. It may be concluded that transport of these potential antigenic compounds occurs directly in lymphocyte-like and macrophage-like cells as well as transport between these two subpopulations.
将大鼠的脾细胞在含有[125I]IgG或[125I]卵清蛋白的伊格尔培养基中孵育10分钟。通过在菲可-泛影酸盐溶液（密度=1.095）中离心分离淋巴细胞样细胞和巨噬细胞样细胞。对照脾细胞在不含[125I]IgG或[125I]卵清蛋白的伊格尔培养基中孵育，并且以类似方式分离这两个亚群。用[125I]IgG或[125I]卵清蛋白标记的淋巴细胞样细胞与对照巨噬细胞样细胞孵育10分钟，并用对照淋巴细胞样细胞标记巨噬细胞样细胞。孵育时间结束后，所有亚群再次在菲可-泛影酸盐溶液中分离。在对照淋巴细胞样细胞和巨噬细胞样细胞的细胞质部分、树液蛋白部分（核质）和染色质中发现了[125I]IgG或[125I]卵清蛋白的放射性。可以得出结论，这些潜在抗原性化合物的转运直接发生在淋巴细胞样细胞和巨噬细胞样细胞中，以及这两个亚群之间的转运。
