Van Rooijen N, Roeterink C H
Immunology. 1980 Apr;39(4):571-6.
The localization of intravenously injected labelled syngeneic lymphoid cells was studied in the spleen of mice and compared with the localization of Indian-ink-containing macrophages. To distinguish between Indian-ink-containing lysosomes of macrophages and silver grains formed in the autoradiographs over the radiolabelled cells, the latter grains were stained blue by a colour-coupling process. Labelled cells were injected 2 h after the Indian ink. Two hours after their injection the bulk of the labelled cells in the spleen was already localized in the white pulp. At this time the Indian ink had been ingested by macrophages in the marginal zone and to a somewhat lesser extent in the red pulp. Twenty-four hours after injection of the cells their concentration in the white pulp appeared constant or had decreased markedly, dependent on the source of the injected cells (spleen, lymph nodes or thymus). At this time carbon-containing macrophages were also found in the white pulp although they contained less carbon than macrophages in the marginal zone and red pulp. A positive correlation between labelled cells and carbon-containing macrophages was never seen in any part of the spleen. It is concluded that, if carbon-containing macrophages induce lymphocyte trapping, as has been supposed by other authors, this trapping must be mediated by the macrophages in an indirect way, e.g. by soluble mediator molecules released in the circulation.
研究了静脉注射标记的同基因淋巴细胞在小鼠脾脏中的定位，并与含印度墨汁的巨噬细胞的定位进行了比较。为了区分巨噬细胞含印度墨汁的溶酶体和放射自显影片上在放射性标记细胞上方形成的银颗粒，通过颜色偶联过程将后者的颗粒染成蓝色。在注射印度墨汁2小时后注射标记细胞。注射后两小时，脾脏中大部分标记细胞已定位在白髓中。此时，印度墨汁已被边缘区的巨噬细胞摄取，红髓中的摄取程度稍低。注射细胞24小时后，它们在白髓中的浓度似乎保持恒定或显著降低，这取决于注射细胞的来源（脾脏、淋巴结或胸腺）。此时，在白髓中也发现了含碳巨噬细胞，尽管它们所含的碳比边缘区和红髓中的巨噬细胞少。在脾脏的任何部位都从未发现标记细胞与含碳巨噬细胞之间存在正相关。得出的结论是，如果如其他作者所推测的那样，含碳巨噬细胞诱导淋巴细胞滞留，那么这种滞留一定是由巨噬细胞以间接方式介导的，例如通过循环中释放的可溶性介质分子。
