Szkudlarek J, Steuden I, Salwa J, Radzikowski C
Arch Immunol Ther Exp (Warsz). 1984;32(6):671-5.
Conventional antisera to L-1210 leukemia were being prepared in our laboratory for nearly a decade and consistently the only specificity detectable was anti Mammary Leukemia antigen (ML). Serological analysis of five monoclonal antibodies obtained following the same immunization schedule showed more diverse pattern of reactivity. Two antigens detected belong to oncofetal category. The third one is differentiation antigen Ly-6 and the nature of two others, expressed on leukemic cells only, remains at present unclear. Thus none of the clones analysed produces antibodies to ML antigen. Our previous analysis of cell surface antigens of L-1210 leukemia with the use of conventional antisera has already been described. This paper presents the results of applying monoclonal antibodies in a comparable studies.
近十年来，我们实验室一直在制备针对L - 1210白血病的传统抗血清，始终检测到的唯一特异性是抗乳腺白血病抗原（ML）。按照相同免疫方案获得的五种单克隆抗体的血清学分析显示出更多样化的反应模式。检测到的两种抗原属于癌胚抗原类别。第三种是分化抗原Ly - 6，另外两种仅在白血病细胞上表达的抗原的性质目前尚不清楚。因此，分析的克隆中没有一个产生针对ML抗原的抗体。我们之前使用传统抗血清对L - 1210白血病细胞表面抗原的分析已经描述过。本文介绍了在类似研究中应用单克隆抗体的结果。
