Balaban C D
Neurosci Res. 1984 Jun;1(3):199-205. doi: 10.1016/s0168-0102(84)80017-6.
This report presents evidence that local injections of 3-acetylpyridine (3-AP) produce circumscribed lesions of the inferior olive (IO) in rabbits. These restricted lesions, assessed in both cresyl violet-stained sections after a 14-day survival and cupric-silver-stained sections after a 16-24 h survival period, are similar in appearance to lesions produced by systemic 3-AP administration in rats. Two types of degeneration are observed: destruction of cells at the injection site and more diffuse cell death in the ipsilateral and contralateral inferior olive. The latter type of degeneration appears to result from IO fiber-of-passage uptake of 3-AP. There was no evidence of lesions in surrounding structures or in sources of axons passing through periolivary regions. Thus, this protocol is a promising tool for studies of climbing fiber connectivity and physiology in rabbits.
本报告提供了证据，表明在兔子身上局部注射3-乙酰吡啶（3-AP）会导致下橄榄核（IO）出现局限性损伤。在存活14天后的甲酚紫染色切片以及存活16 - 24小时后的铜银染色切片中评估的这些局限性损伤，其外观与大鼠全身注射3-AP所产生的损伤相似。观察到两种类型的变性：注射部位细胞的破坏以及同侧和对侧下橄榄核中更弥漫性的细胞死亡。后一种类型的变性似乎是由于3-AP被下橄榄核的传入纤维摄取所致。没有证据表明周围结构或穿过橄榄周区域的轴突来源存在损伤。因此，该方案是研究兔子攀爬纤维连接性和生理学的一种有前景的工具。
