Espmark A, Möller E
Tissue Antigens. 1978 Mar;11(3):273-8. doi: 10.1111/j.1399-0039.1978.tb01258.x.
Skin fibroblasts derived from persons who had been HLA-typed by conventional lymphocytotoxocity tests were subjected to HLA-typing by the mixed hemadsorption (MH) test. The agreement between the two methods was good, provided sera for the MH test were carefully selected. Minor discrepancies found in this study can probably be corrected by further serum selection. There was no difficulty in typing late passages of cultured diploid cells (22 doublings) and in fact it was easier to type a culture at the 33rd doubling than at the 11th. Thus no signs of disappearance of HLA-antigens were seen in these tests.
通过传统淋巴细胞毒性试验进行HLA分型的人的皮肤成纤维细胞，采用混合血细胞吸附（MH）试验进行HLA分型。如果仔细选择用于MH试验的血清，两种方法之间的一致性良好。本研究中发现的微小差异可能通过进一步的血清选择得到纠正。对培养的二倍体细胞的后期传代（22次倍增）进行分型没有困难，实际上对第33次倍增的培养物进行分型比第11次倍增时更容易。因此，在这些试验中未观察到HLA抗原消失的迹象。
