Ostertag W, Freshney M, Vehmeyer K, Jasmin C, Rutter G
J Virol. 1984 Jan;49(1):253-61. doi: 10.1128/JVI.49.1.253-261.1984.
The myeloproliferative sarcoma virus is molecularly related to the Moloney sarcoma virus (Pragnell et al., J. Virol. 38:952-957, 1981), but causes both fibroblast transformation in vitro and leukemic changes--including spleen focus formation--in adult mice. The fibroblast transforming properties of myeloproliferative sarcoma virus were used to select viral temperature-sensitive mutants at 39.5 degrees C, the nonpermissive temperature. These mutants are temperature sensitive in the maintenance of the transformed state. This was also shown by cytoskeletal changes of the infected cells at permissive and nonpermissive temperatures. Viruses released from cells maintained at both the permissive and nonpermissive temperature are temperature sensitive in fibroblast transformation functions. All temperature-sensitive mutants show only a low reversion rate to wild-type transforming function. The myeloproliferative sarcoma virus temperature-sensitive mutants are inefficient in causing leukemic transformation (spleen enlargement, focus formation) in mice at the normal temperature. A method to maintain a low body temperature (33 to 34 degrees C) in mice is described. One temperature-sensitive mutant was checked at low body temperature and did not induce leukemia. These data thus indicate that the same or related viral functions are responsible for hematopoietic and fibroblast transformation.
骨髓增殖性肉瘤病毒在分子水平上与莫洛尼肉瘤病毒相关（普拉格内尔等人，《病毒学杂志》38:952 - 957，1981），但在体外可引起成纤维细胞转化，并在成年小鼠中引发白血病变化，包括脾灶形成。利用骨髓增殖性肉瘤病毒的成纤维细胞转化特性，在非允许温度39.5℃下筛选病毒温度敏感突变体。这些突变体在维持转化状态方面对温度敏感。在允许温度和非允许温度下感染细胞的细胞骨架变化也证明了这一点。在允许温度和非允许温度下培养的细胞释放的病毒在成纤维细胞转化功能方面对温度敏感。所有温度敏感突变体向野生型转化功能的回复率都很低。骨髓增殖性肉瘤病毒温度敏感突变体在正常温度下在小鼠中引发白血病转化（脾肿大、灶形成）的效率较低。描述了一种在小鼠中维持低体温（33至34℃）的方法。在低体温下检测了一个温度敏感突变体，它没有诱发白血病。因此，这些数据表明相同或相关的病毒功能负责造血和成纤维细胞转化。
