Sehgal P B, Inoue M, Tan Y H
J Interferon Res. 1984;4(1):63-6. doi: 10.1089/jir.1984.4.63.
Does the human "beta 2 interferon" (HuIFN-beta 2) mRNA of length 1.3 kb obtained from poly(I) X poly(C) and cycloheximide-induced human diploid fibroblasts (FS-4) code for an interferon per se, or for a protein which, in turn, induces IFN-beta in the cell culture assay that is usually used to detect the antiviral activity of the translation product (obtained from Xenopus laevis oocytes) of this mRNA? This question has been resolved by demonstrating that the IFN derived from the 1.3 kb mRNA can be detected by the direct assay of the oocyte translation product in a highly sensitive radioimmunoassay based on a high-affinity polyclonal antibody raised against N-terminal aminoacid sequence pure IFN-beta 1.
从聚肌苷酸×聚胞苷酸（poly(I)×poly(C)）和放线菌酮诱导的人二倍体成纤维细胞（FS-4）中获得的长度为1.3 kb的人“β2干扰素”（HuIFN-β2）mRNA，编码的是一种干扰素本身，还是编码一种蛋白质，而这种蛋白质反过来又能在通常用于检测该mRNA翻译产物（从非洲爪蟾卵母细胞获得）抗病毒活性的细胞培养试验中诱导IFN-β？通过证明基于针对N端氨基酸序列纯IFN-β1产生的高亲和力多克隆抗体，在高度灵敏的放射免疫分析中直接检测卵母细胞翻译产物，能够检测到源自1.3 kb mRNA的IFN，这个问题得到了解决。
