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1
Does 3'-terminal poly(A) stabilize human fibroblast interferon mRNA in oocytes of Xenopus laevis?3'末端的聚腺苷酸（poly(A)）是否能稳定非洲爪蟾卵母细胞中的人成纤维细胞干扰素mRNA？
Proc Natl Acad Sci U S A. 1978 Oct;75(10):5030-3. doi: 10.1073/pnas.75.10.5030.
2
Translational activity and functional stability of human fibroblast beta 1 and beta 2 interferon mRNAs lacking 3'-terminal RNA sequences.缺乏3'-末端RNA序列的人成纤维细胞β1和β2干扰素mRNA的翻译活性和功能稳定性。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1741-5. doi: 10.1073/pnas.78.3.1741.
3
Interferon messenger RNA content of human fibroblasts during induction, shutoff, and superinduction of interferon production.人成纤维细胞在干扰素产生的诱导、关闭和超诱导过程中的干扰素信使核糖核酸含量。
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3409-13. doi: 10.1073/pnas.74.8.3409.
4
Globin mRNA species containing poly(A) segments of different lengths. Their functional stability in Xenopus oocytes.含有不同长度聚腺苷酸（poly(A)）片段的珠蛋白信使核糖核酸（mRNA）种类。它们在非洲爪蟾卵母细胞中的功能稳定性。
Eur J Biochem. 1976 Apr 15;64(1):115-21. doi: 10.1111/j.1432-1033.1976.tb10279.x.
5
Induction and decay of human fibroblast interferon mRNA.人成纤维细胞干扰素mRNA的诱导与衰减
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4415-9. doi: 10.1073/pnas.74.10.4415.
6
Synthesis of interferon in heterologous cells, cell-free extracts, and Xenopus laevis oocytes.干扰素在异源细胞、无细胞提取物及非洲爪蟾卵母细胞中的合成。
Tex Rep Biol Med. 1977;35:117-25.
7
Synthesis of human interferon by Xenopus laevis oocytes: two structural genes for interferons in human cells.非洲爪蟾卵母细胞合成人干扰素：人类细胞中干扰素的两个结构基因。
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3287-91. doi: 10.1073/pnas.74.8.3287.
8
Interferon activity produced by translation of human interferon messenger RNA in cell-free ribosomal systems and in Xenopus oöcytes.在无细胞核糖体系统和非洲爪蟾卵母细胞中通过人干扰素信使核糖核酸的翻译所产生的干扰素活性。
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4881-5. doi: 10.1073/pnas.72.12.4881.
9
Regulation of the stability of poly(I)xpoly(C)-induced human fibroblast interferon mRNA: selective inactivation of interferon mRNA and lack of involvement of 2',5'-oligo(A) synthetase activation during the shutoff of interferon production.聚肌苷酸×聚胞苷酸诱导的人成纤维细胞干扰素mRNA稳定性的调节：干扰素mRNA的选择性失活以及在干扰素产生关闭过程中2',5'-寡聚腺苷酸合成酶激活未参与其中。
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3489-93. doi: 10.1073/pnas.77.6.3489.
10
Relationship between interferon production and interferon messenger RNA synthesis in human fibroblasts.人成纤维细胞中干扰素产生与干扰素信使核糖核酸合成之间的关系。
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1483-7. doi: 10.1073/pnas.74.4.1483.

引用本文的文献

1
Regulation of heme oxygenase-1 mRNA deadenylation and turnover in NIH3T3 cells by nitrosative or alkylation stress.亚硝化或烷基化应激对NIH3T3细胞中血红素加氧酶-1 mRNA去腺苷酸化和周转的调控
BMC Mol Biol. 2007 Dec 20;8:116. doi: 10.1186/1471-2199-8-116.
2
Translational stability of native and deadenylylated rabbit globin mRNA injected into HeLa cells.注射到HeLa细胞中的天然和去腺苷酸化兔珠蛋白mRNA的翻译稳定性。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):908-11. doi: 10.1073/pnas.78.2.908.
3
Post-transcriptional modification of the poly(A) length of galactose-1-phosphate uridyl transferase mRNA in Saccharomyces cerevisiae.酿酒酵母中1-磷酸半乳糖尿苷酰转移酶mRNA的聚腺苷酸长度的转录后修饰
Nucleic Acids Res. 1980 Sep 11;8(17):3841-9. doi: 10.1093/nar/8.17.3841.
4
Simian virus 40 cRNA is processed into functional mRNA in microinjected monkey cells.猿猴病毒40的互补RNA（cRNA）在显微注射的猴细胞中被加工成功能性信使核糖核酸（mRNA）。
EMBO J. 1982;1(9):1081-8. doi: 10.1002/j.1460-2075.1982.tb01300.x.
5
Translational activity and functional stability of human fibroblast beta 1 and beta 2 interferon mRNAs lacking 3'-terminal RNA sequences.缺乏3'-末端RNA序列的人成纤维细胞β1和β2干扰素mRNA的翻译活性和功能稳定性。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1741-5. doi: 10.1073/pnas.78.3.1741.
6
Possible involvement of poly(A) in protein synthesis.聚腺苷酸（poly(A)）可能参与蛋白质合成。
Nucleic Acids Res. 1983 Sep 24;11(18):6353-68. doi: 10.1093/nar/11.18.6353.
7
The effect of capping and polyadenylation on the stability, movement and translation of synthetic messenger RNAs in Xenopus oocytes.加帽和聚腺苷酸化对非洲爪蟾卵母细胞中合成信使核糖核酸的稳定性、移动性和翻译的影响。
Nucleic Acids Res. 1985 Oct 25;13(20):7375-94. doi: 10.1093/nar/13.20.7375.
8
Relatively stable population of c-myc RNA that lacks long poly(A).缺乏长聚腺苷酸尾的相对稳定的c-myc RNA群体。
Mol Cell Biol. 1987 Jun;7(6):2052-8. doi: 10.1128/mcb.7.6.2052-2058.1987.
9
Determinants of mRNA stability in Dictyostelium discoideum amoebae: differences in poly(A) tail length, ribosome loading, and mRNA size cannot account for the heterogeneity of mRNA decay rates.盘基网柄菌变形虫中mRNA稳定性的决定因素：多聚腺苷酸尾长度、核糖体负载和mRNA大小的差异无法解释mRNA衰减率的异质性。
Mol Cell Biol. 1988 May;8(5):1957-69. doi: 10.1128/mcb.8.5.1957-1969.1988.
10
Stability and movement of mRNAs and their encoded proteins in Xenopus oocytes.非洲爪蟾卵母细胞中mRNA及其编码蛋白质的稳定性与运动
J Cell Biol. 1985 Apr;100(4):1148-56. doi: 10.1083/jcb.100.4.1148.

本文引用的文献

1
Amino acid metabolism in mammalian cell cultures.哺乳动物细胞培养中的氨基酸代谢
Science. 1959 Aug 21;130(3373):432-7. doi: 10.1126/science.130.3373.432.
2
Regulation of transcription of the Reovirus genome.呼肠孤病毒基因组转录的调控
J Mol Biol. 1968 Aug 28;36(1):107-23. doi: 10.1016/0022-2836(68)90223-4.
3
Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.在琼脂糖-丙烯酰胺复合凝胶中通过电泳进行核糖核酸的分子量估计及分离
Biochemistry. 1968 Feb;7(2):668-74. doi: 10.1021/bi00842a023.
4
Production of high-titered interferon in cultures of human diploid cells.在人二倍体细胞培养物中产生高滴度干扰素。
Antimicrob Agents Chemother. 1972 Dec;2(6):476-84. doi: 10.1128/AAC.2.6.476.
5
In vitro translation of polyadenylic acid-free rabbit globin messenger RNA.无聚腺苷酸兔珠蛋白信使核糖核酸的体外翻译
J Mol Biol. 1974 Sep 5;88(1):233-45. doi: 10.1016/0022-2836(74)90307-6.
6
Role of the polyadenylate segment in the translation of globin messenger RNA in Xenopus oocytes.聚腺苷酸片段在非洲爪蟾卵母细胞中珠蛋白信使核糖核酸翻译中的作用。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3143-6. doi: 10.1073/pnas.71.8.3143.
7
Semi-micro, dye-binding assay for rabbit interferon.兔干扰素的半微量染料结合测定法。
Appl Microbiol. 1971 Apr;21(4):723-5. doi: 10.1128/am.21.4.723-725.1971.
8
Further evidence on the nuclear origin and transfer to the cytoplasm of polyadenylic acid sequences in mammalian cell RNA.关于哺乳动物细胞RNA中多聚腺苷酸序列的核起源及其向细胞质转移的进一步证据。
J Mol Biol. 1973 Apr 15;75(3):515-32. doi: 10.1016/0022-2836(73)90458-0.
9
Action of dichlorobenzimidazole riboside on RNA synthesis in L-929 and HeLa cells.二氯苯并咪唑核糖苷对L-929和HeLa细胞中RNA合成的作用。
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10
Human interferon production: superinduction by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole.人类干扰素的产生：5,6-二氯-1-β-D-呋喃核糖基苯并咪唑的超诱导作用。
Science. 1975 Oct 17;190(4211):282-4. doi: 10.1126/science.1179208.

3'末端的聚腺苷酸（poly(A)）是否能稳定非洲爪蟾卵母细胞中的人成纤维细胞干扰素mRNA？

Does 3'-terminal poly(A) stabilize human fibroblast interferon mRNA in oocytes of Xenopus laevis?

作者信息

Sehgal P B, Soreq H, Tamm I

出版信息

Proc Natl Acad Sci U S A. 1978 Oct;75(10):5030-3. doi: 10.1073/pnas.75.10.5030.

DOI:10.1073/pnas.75.10.5030
PMID:283411
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336256/
Abstract

Polynucleotide phosphorylase (polyribonucleotide:orthophosphate nucleotidyltransferase, EC 2.7.7.8) purified from Escherichia coli was used enzymatically to deadenylate polyadenylated human fibroblast interferon mRNA preparations obtained from human diploid fibroblasts (FS-4 strain) induced by poly(I)-poly(C) (20 microgram/ml) in the presence of cycloheximide (50 microgram/ml, 4 hr). Both the polyadenylated and the deadenylated interferon mRNA preparations were translated into biologically active human interferon when injected into oocytes of Xenopus laevis. In the oocytes the functional stability of deadenylated interferon mRNA was indistinguishable from that of polyadenylated interferon mRNA.

摘要

从大肠杆菌中纯化得到的多核苷酸磷酸化酶（聚核糖核苷酸：正磷酸核苷酸基转移酶，EC 2.7.7.8）被用于酶促反应，使从人二倍体成纤维细胞（FS-4株）中获得的聚腺苷酸化人成纤维细胞干扰素mRNA制剂去腺苷酸化。这些人二倍体成纤维细胞由聚（I）-聚（C）（20微克/毫升）在环己酰亚胺（50微克/毫升，4小时）存在的情况下诱导产生。当将聚腺苷酸化和去腺苷酸化的干扰素mRNA制剂注射到非洲爪蟾的卵母细胞中时，二者均可被翻译成具有生物活性的人干扰素。在卵母细胞中，去腺苷酸化的干扰素mRNA的功能稳定性与聚腺苷酸化的干扰素mRNA的功能稳定性没有差异。