一种用于透射电子显微镜的无包埋切片制备新方法：应用于细胞骨架框架及其他三维网络
A new method of preparing embeddment-free sections for transmission electron microscopy: applications to the cytoskeletal framework and other three-dimensional networks.
作者信息
Capco D G, Krochmalnic G, Penman S
出版信息
J Cell Biol. 1984 May;98(5):1878-85. doi: 10.1083/jcb.98.5.1878.
Abstract
Diethylene glycol distearate is used as a removable embedding medium to produce embeddment -free sections for transmission electron microscopy. The easily cut sections of this material float and form ribbons in a water-filled knife trough and exhibit interference colors that aid in the selection of sections of equal thickness. The images obtained with embeddment -free sections are compared with those from the more conventional epoxy-embedded sections, and illustrate that embedding medium can obscure important biological structures, especially protein filament networks. The embeddment -free section methodology is well suited for morphological studies of cytoskeletal preparations obtained by extraction of cells with nonionic detergent in cytoskeletal stabilizing medium. The embeddment -free section also serves to bridge the very different images afforded by embedded sections and unembedded whole mounts.
摘要
二乙二醇二硬脂酸酯用作可去除的包埋介质，以制备用于透射电子显微镜的无包埋切片。这种材料易于切割的切片在充满水的刀槽中漂浮并形成带状，呈现干涉色，有助于选择等厚的切片。将无包埋切片获得的图像与更传统的环氧树脂包埋切片的图像进行比较，结果表明包埋介质可能会掩盖重要的生物结构，尤其是蛋白质丝网络。无包埋切片方法非常适合对在细胞骨架稳定介质中用非离子去污剂提取细胞所获得的细胞骨架制剂进行形态学研究。无包埋切片还用于弥合包埋切片和未包埋整装片所提供的非常不同的图像之间的差距。
相似文献
1
A new method of preparing embeddment-free sections for transmission electron microscopy: applications to the cytoskeletal framework and other three-dimensional networks.一种用于透射电子显微镜的无包埋切片制备新方法：应用于细胞骨架框架及其他三维网络
J Cell Biol. 1984 May;98(5):1878-85. doi: 10.1083/jcb.98.5.1878.
2
Immunolocalization in three dimensions: immunogold staining of cytoskeletal and nuclear matrix proteins in resinless electron microscopy sections.三维免疫定位：无树脂电子显微镜切片中细胞骨架和核基质蛋白的免疫金染色
Proc Natl Acad Sci U S A. 1990 Mar;87(6):2259-63. doi: 10.1073/pnas.87.6.2259.
3
Resinless section electron microscopy of HeLa cell mitotic architecture.HeLa细胞有丝分裂结构的无树脂切片电子显微镜观察
Proc Natl Acad Sci U S A. 1986 Dec;83(23):8996-9000. doi: 10.1073/pnas.83.23.8996.
4
Evidence of a microtrabecular cytoskeletal lattice in glandular cells of hydrozoan planulae.
J Morphol. 1986 Mar;187(3):353-362. doi: 10.1002/jmor.1051870307.
5
Epithelial cytoskeletal framework and nuclear matrix-intermediate filament scaffold: three-dimensional organization and protein composition.上皮细胞骨架框架与核基质-中间丝支架：三维结构与蛋白质组成
J Cell Biol. 1984 Jun;98(6):1973-84. doi: 10.1083/jcb.98.6.1973.
6
Cytoskeletal reorganization during early mammalian development: analysis using embedment-free sections.
Dev Biol. 1986 Jun;115(2):446-58. doi: 10.1016/0012-1606(86)90265-4.
7
Detection of fibrils associated with Rickettsia rickettsii.与立氏立克次体相关的纤丝的检测。
Infect Immun. 1983 Sep;41(3):1252-60. doi: 10.1128/iai.41.3.1252-1260.1983.
8
The cytoplasmic filament system in critical point-dried whole mounts and plastic-embedded sections.临界点干燥整装标本和塑料包埋切片中的细胞质细丝系统。
J Cell Biol. 1985 May;100(5):1474-87. doi: 10.1083/jcb.100.5.1474.
9
Epithelial structure revealed by chemical dissection and unembedded electron microscopy.通过化学剖析和未包埋电子显微镜揭示的上皮结构。
J Cell Biol. 1984 Jul;99(1 Pt 2):203s-208s. doi: 10.1083/jcb.99.1.203s.
10
Ultrastructural filament organization of hepatocyte cytoplasm revealed by embedment-free sections of rat liver.
J Gastroenterol Hepatol. 1993 Sep-Oct;8(5):451-6. doi: 10.1111/j.1440-1746.1993.tb01547.x.
引用本文的文献
1
Follicle cell development is partly independent of germ-line cell differentiation in Drosophila oogenesis.在果蝇卵子发生过程中，卵泡细胞的发育部分独立于生殖系细胞的分化。
Rouxs Arch Dev Biol. 1989 Nov;198(4):185-190. doi: 10.1007/BF00375904.
2
An architectural genetic and epigenetic perspective.建筑的遗传与表观遗传学视角。
Integr Biol (Camb). 2011 Apr;3(4):297-303. doi: 10.1039/c0ib00103a. Epub 2010 Dec 24.
3
Nucleoskeleton of early bovine embryos and differentiated somatic cells: an ultrastructural and immunocytochemical comparison.早期牛胚胎和分化体细胞的核骨架：超微结构和免疫细胞化学比较
Histochem Cell Biol. 2004 Jun;121(6):441-51. doi: 10.1007/s00418-004-0655-3. Epub 2004 May 25.
4
Amino-terminal polypeptides of vimentin are responsible for the changes in nuclear architecture associated with human immunodeficiency virus type 1 protease activity in tissue culture cells.波形蛋白的氨基末端多肽负责组织培养细胞中与1型人类免疫缺陷病毒蛋白酶活性相关的核结构变化。
Mol Biol Cell. 2001 Jan;12(1):143-54. doi: 10.1091/mbc.12.1.143.
5
Half a century of "the nuclear matrix".“核基质”的半个世纪
Mol Biol Cell. 2000 Mar;11(3):799-805. doi: 10.1091/mbc.11.3.799.
6
The nuclear matrix prepared by amine modification.通过胺修饰制备的核基质。
Proc Natl Acad Sci U S A. 1999 Feb 2;96(3):933-8. doi: 10.1073/pnas.96.3.933.
7
Communication between the cell membrane and the nucleus: role of protein compartmentalization.细胞膜与细胞核之间的通讯：蛋白质区室化的作用。
J Cell Biochem Suppl. 1998;30-31:250-63.
8
Structural protein 4.1 in the nucleus of human cells: dynamic rearrangements during cell division.人类细胞核中的结构蛋白4.1：细胞分裂过程中的动态重排
J Cell Biol. 1997 Apr 21;137(2):275-89. doi: 10.1083/jcb.137.2.275.
9
The nuclear matrix revealed by eluting chromatin from a cross-linked nucleus.通过从交联核中洗脱染色质而揭示的核基质。
Proc Natl Acad Sci U S A. 1997 Apr 29;94(9):4446-50. doi: 10.1073/pnas.94.9.4446.
10
Resinless section electron microscopy reveals the yeast cytoskeleton.无树脂切片电子显微镜揭示酵母细胞骨架。
Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):3732-5. doi: 10.1073/pnas.94.8.3732.
本文引用的文献
1
PENETRATION OF STAIN IN ULTRATHIN SECTIONS OF TOBACCO MOSAIC VIRUS.烟草花叶病毒超薄切片中染色剂的渗透
Stain Technol. 1964 Sep;39:257-65. doi: 10.3109/10520296409061241.
2
DIETHYLENE GLYCOL DISTEARATE EMBEDDING AND ULTRAMICROTOME SECTIONING FOR LIGHT MICROSCOPY.
Stain Technol. 1964 Jan;39:13-7. doi: 10.3109/10520296409061202.
3
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中，将高pH值的柠檬酸铅用作电子不透明染色剂。
J Cell Biol. 1963 Apr;17(1):208-12. doi: 10.1083/jcb.17.1.208.
4
The application of polyethylene glycol (PEG) to electron microscopy.聚乙二醇（PEG）在电子显微镜中的应用。
J Cell Biol. 1980 Aug;86(2):675-61. doi: 10.1083/jcb.86.2.675.
5
The nuclear matrix: three-dimensional architecture and protein composition.核基质：三维结构与蛋白质组成
Cell. 1982 Jul;29(3):847-58. doi: 10.1016/0092-8674(82)90446-9.
6
Improved diethylene glycol distearate embedding wax.
Stain Technol. 1982 Jan;57(1):39-43. doi: 10.3109/10520298209066518.
7
Actin gene expression visualized in chicken muscle tissue culture by using in situ hybridization with a biotinated nucleotide analog.通过使用与生物素化核苷酸类似物的原位杂交技术，在鸡肌肉组织培养物中观察到肌动蛋白基因的表达。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7331-5. doi: 10.1073/pnas.79.23.7331.
8
Cytoplasmic and nuclear architecture in cells and tissue: form, functions, and mode of assembly.细胞与组织中的细胞质和细胞核结构：形态、功能及组装方式
Cold Spring Harb Symp Quant Biol. 1982;46 Pt 2:1013-28. doi: 10.1101/sqb.1982.046.01.094.
9
Filament organization revealed in platinum replicas of freeze-dried cytoskeletons.在冻干细胞骨架的铂复制品中揭示的细丝组织。
J Cell Biol. 1980 Jul;86(1):212-34. doi: 10.1083/jcb.86.1.212.
10
Production of monoclonal antibodies against calmodulin by in vitro immunization of spleen cells.通过脾细胞体外免疫制备抗钙调蛋白的单克隆抗体。
J Cell Biol. 1983 Apr;96(4):1149-54. doi: 10.1083/jcb.96.4.1149.
Zotero 插件