Verbueken A H, Van Grieken R E, Paulus G J, Verpooten G A, De Broe M E
Biomed Mass Spectrom. 1984 Apr;11(4):159-63. doi: 10.1002/bms.1200110404.
By means of laser microprobe mass analysis ( LAMMA ) platinum was detected in the renal proximal tubular cells of a dog that had been intravenously administered the antitumor drug cisplatin (5 mg per kg body weight). No definite subcellular localization of the heavy metal was obtained. Sample preparation and analytical features are examined to increase spatial resolution of analysis while maintaining sufficient detection efficiency. The LAMMA method is destructive, but the amount and type of evaporated material can readily be determined when using LAMMA in combination with transmission electron microscopy. Instrumental optimization and standardization of mass signals is possible by using platinum-loaded, ion-chelating resin beads embedded and sectioned with the tissue.
通过激光微探针质谱分析（LAMMA），在一只静脉注射了抗肿瘤药物顺铂（每千克体重5毫克）的狗的肾近端小管细胞中检测到了铂。未获得重金属明确的亚细胞定位。对样品制备和分析特征进行了研究，以在保持足够检测效率的同时提高分析的空间分辨率。LAMMA方法具有破坏性，但当将LAMMA与透射电子显微镜结合使用时，可以很容易地确定蒸发物质的数量和类型。通过使用嵌入组织并切片的负载铂的离子螯合树脂珠，可以实现仪器的优化和质量信号的标准化。
