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本文引用的文献

1
Response of Chinese hamster ovary cells to anticancer drugs under aerobic and hypoxic conditions.中国仓鼠卵巢细胞在有氧和缺氧条件下对抗癌药物的反应。
Br J Cancer. 1981 Feb;43(2):245-8. doi: 10.1038/bjc.1981.37.
2
Response of cultured human brain tumors to nitrosoureas: correlation with clinical data.培养的人脑肿瘤对亚硝基脲类药物的反应：与临床数据的相关性。
Cancer. 1981 Jan 15;47(2):255-65. doi: 10.1002/1097-0142(19810115)47:2<255::aid-cncr2820470209>3.0.co;2-j.
3
Classification of antineoplastic agents by their selective toxicities toward oxygenated and hypoxic tumor cells.根据抗肿瘤药物对富氧和缺氧肿瘤细胞的选择性毒性进行分类。
Cancer Res. 1981 Jan;41(1):73-81.
4
Cytotoxicity of adriamycin on aerobic and hypoxic chinese hamster V79 cells in vitro.阿霉素对体外培养的需氧和缺氧中国仓鼠V79细胞的细胞毒性。
Br J Cancer. 1980 Oct;42(4):568-73. doi: 10.1038/bjc.1980.281.
5
Human tumour karyology: marked analytic improvement by short-term agar culture.人类肿瘤细胞遗传学：短期琼脂培养显著提高分析效果。
Br J Cancer. 1980 Jun;41(6):867-74. doi: 10.1038/bjc.1980.162.
6
The poor cloning ability in agar of human tumour cells from biopsies of primary tumours.
Cell Biol Int Rep. 1980 May;4(5):479-86. doi: 10.1016/0309-1651(80)90035-1.
7
Quantitative association between the in vitro human tumor stem cell assay and clinical response to cancer chemotherapy.体外人肿瘤干细胞检测与癌症化疗临床反应之间的定量关联。
Cancer Chemother Pharmacol. 1981;6(3):211-8. doi: 10.1007/BF00256973.
8
Comparison of two soft-agar methods for assaying chemosensitivity of human tumours in vitro: malignant melanomas.两种体外检测人类肿瘤（恶性黑色素瘤）化学敏感性的软琼脂方法的比较
Br J Cancer. 1981 Oct;44(4):539-44. doi: 10.1038/bjc.1981.223.
9
Cancer drug sensitivity test has problems.癌症药物敏感性测试存在问题。
JAMA. 1982 Dec 17;248(23):3079, 3083-4. doi: 10.1001/jama.248.23.3079.
10
Effect of oxygen concentration on the growth and drug sensitivity of human melanoma cells in soft-agar clonogenic assay.在软琼脂克隆形成试验中氧浓度对人黑色素瘤细胞生长和药物敏感性的影响
Cancer Res. 1982 Mar;42(3):1005-7.

软琼脂试验中肿瘤集落生长的切片分析：氧气的影响

Sectional analysis of tumor colony growth in the soft-agar assay: effects of oxygen.

作者信息

Fan D, Fan S, Morgan L R

出版信息

J Cancer Res Clin Oncol. 1984;108(2):181-5. doi: 10.1007/BF00402464.

DOI:10.1007/BF00402464
PMID:6540780
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12252633/
Abstract

Soft-agar clonogenicity of L1210 mouse leukemia cells and of xenografts of a human melanoma and a carcinoma of the cervix was studied sectionally by the sizes of the colonies grown under hypoxic gradients and aerobic condition. Soft-agar plating efficiency was increased in cultured L1210 cells with decreasing oxygen concentrations. The growth of both cultured L1210 cells and their BDF1 ascites was better in 5% oxygen than in 20% oxygen. Although soft-agar colony development of both melanoma and cervical carcinoma was significantly better in 5% oxygen, the former has a secondary preference for a hypoxic atmosphere and the latter, for an aerobic condition.

摘要

通过在缺氧梯度和有氧条件下生长的集落大小，对L1210小鼠白血病细胞、人黑色素瘤异种移植物和子宫颈癌进行了软琼脂克隆形成能力的分段研究。随着氧浓度降低，培养的L1210细胞的软琼脂接种效率增加。培养的L1210细胞及其BDF1腹水在5%氧气中的生长比在20%氧气中更好。尽管黑色素瘤和子宫颈癌在5%氧气中的软琼脂集落形成明显更好，但前者对缺氧环境有次要偏好，而后者则偏好有氧条件。