Sectional analysis of tumor colony growth in the soft-agar assay: effects of oxygen.
作者信息
Fan D, Fan S, Morgan L R
出版信息
J Cancer Res Clin Oncol. 1984;108(2):181-5. doi: 10.1007/BF00402464.
Abstract
Soft-agar clonogenicity of L1210 mouse leukemia cells and of xenografts of a human melanoma and a carcinoma of the cervix was studied sectionally by the sizes of the colonies grown under hypoxic gradients and aerobic condition. Soft-agar plating efficiency was increased in cultured L1210 cells with decreasing oxygen concentrations. The growth of both cultured L1210 cells and their BDF1 ascites was better in 5% oxygen than in 20% oxygen. Although soft-agar colony development of both melanoma and cervical carcinoma was significantly better in 5% oxygen, the former has a secondary preference for a hypoxic atmosphere and the latter, for an aerobic condition.
摘要
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