软琼脂试验中肿瘤集落生长的切片分析：氧气的影响
Sectional analysis of tumor colony growth in the soft-agar assay: effects of oxygen.
作者信息
Fan D, Fan S, Morgan L R
出版信息
J Cancer Res Clin Oncol. 1984;108(2):181-5. doi: 10.1007/BF00402464.
Abstract
Soft-agar clonogenicity of L1210 mouse leukemia cells and of xenografts of a human melanoma and a carcinoma of the cervix was studied sectionally by the sizes of the colonies grown under hypoxic gradients and aerobic condition. Soft-agar plating efficiency was increased in cultured L1210 cells with decreasing oxygen concentrations. The growth of both cultured L1210 cells and their BDF1 ascites was better in 5% oxygen than in 20% oxygen. Although soft-agar colony development of both melanoma and cervical carcinoma was significantly better in 5% oxygen, the former has a secondary preference for a hypoxic atmosphere and the latter, for an aerobic condition.
摘要
通过在缺氧梯度和有氧条件下生长的集落大小，对L1210小鼠白血病细胞、人黑色素瘤异种移植物和子宫颈癌进行了软琼脂克隆形成能力的分段研究。随着氧浓度降低，培养的L1210细胞的软琼脂接种效率增加。培养的L1210细胞及其BDF1腹水在5%氧气中的生长比在20%氧气中更好。尽管黑色素瘤和子宫颈癌在5%氧气中的软琼脂集落形成明显更好，但前者对缺氧环境有次要偏好，而后者则偏好有氧条件。
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