Belin D, Godeau F, Vassalli J D
EMBO J. 1984 Aug;3(8):1901-6. doi: 10.1002/j.1460-2075.1984.tb02065.x.
In mouse MSV-3T3 cells the synthesis of the urokinase form of plasminogen activator was increased 10-fold after addition of the tumor promoter phorbol-12-myristate-13-acetate (PMA). PMA also stimulated the secretion of the protein into the culture medium, mostly in the form of enzymatically inactive pro-urokinase. When assayed by injecting RNA into Xenopus laevis oocytes, the concentration of functional urokinase mRNA was found to be 6- to 10-fold higher in the PMA-treated cells; a similar increase in urokinase mRNA content was measured by hybridisation with a mouse urokinase cDNA probe. Thus, the induction of plasminogen activator by PMA in MSV-3T3 cells is accounted for by an increased content of urokinase mRNA.
在小鼠MSV - 3T3细胞中，加入肿瘤促进剂佛波醇 - 12 - 肉豆蔻酸酯 - 13 - 乙酸酯（PMA）后，尿激酶型纤溶酶原激活剂的合成增加了10倍。PMA还刺激该蛋白分泌到培养基中，大多以无酶活性的尿激酶原形式存在。通过将RNA注射到非洲爪蟾卵母细胞中进行检测时，发现经PMA处理的细胞中功能性尿激酶mRNA的浓度高6至10倍；用小鼠尿激酶cDNA探针杂交测量，尿激酶mRNA含量也有类似增加。因此，PMA对MSV - 3T3细胞中纤溶酶原激活剂的诱导作用是由尿激酶mRNA含量增加所致。
