Experimental brain tumors and edema in rats. II. Tumor edema.

Experimentally induced brain tumors in rats with the morphological characteristics of low differentiated gliomas were investigated with special consideration of the distribution of extravasated serum proteins within the tumor itself. Protein extravasation across tumor vessels occurs by pinocytotic vesicles which enclose serum proteins at the luminal surface of the endothelial cells. These vesicles move to the abluminal cell surface where proteins are released after fusion between vesicles and the cell membrane. Serum proteins then spread through the extracellular space from where they are taken up in tumor cells by pinocytosis. They are then transmitted to and digested by lysosomes in which acid phosphatase activity could be demonstrated. Tracer studies (human serum albumin, horseradish peroxidase) revealed that proteins reach the lysosomes 15 min after systemic application. The cellular content of tracers is variable even when systemic circulation has lasted 120 min.