Lizana J, Olsson I
J Clin Chem Clin Biochem. 1984 Aug;22(8):545-9. doi: 10.1515/cclm.1984.22.8.545.
A routine method for the analysis of Vitamin D-binding protein (Gc-globulin) phenotypes has been developed. The assay is based on isoelectric focusing in a narrow pH interval (4.5-5.4) using Agarose IEF (1%) or polyacrylamide (T5% C3%) gels, followed by immunofixation. When focused in this pH gradient the anodal and cathodal bands of the phenotype Gc 1-2 are separately by 16-18 mm. The "fast" and "slow" bands of the phenotype Gc 1-1 can also be easily distinguished from each other. The isoelectric focusing technique which has been developed resolves the Vitamin D-binding protein phenotypes in a fast, convenient and reproducible manner. Since agarose gels are so easy to cast and process, Agarose IEF is recommended as the matrix of choice.
已开发出一种分析维生素 D 结合蛋白（Gc 球蛋白）表型的常规方法。该测定基于在狭窄 pH 区间（4.5 - 5.4）使用琼脂糖等电聚焦（1%）或聚丙烯酰胺（T5% C3%）凝胶进行等电聚焦，随后进行免疫固定。当在该 pH 梯度中聚焦时，Gc 1 - 2 表型的阳极带和阴极带相隔 16 - 18 毫米。Gc 1 - 1 表型的“快”带和“慢”带也能很容易地相互区分。所开发的等电聚焦技术能以快速、便捷且可重复的方式解析维生素 D 结合蛋白表型。由于琼脂糖凝胶易于制备和处理，推荐使用琼脂糖等电聚焦作为首选基质。
