Jin Z, Wu M
Hua Xi Yi Ke Da Xue Xue Bao. 1993 Sep;24(3):281-2.
The group-specific component (GC) is an alpha 2 globulin with high genetic polymorphism in serum. There is also a little amount of GC in human semen. Pflug and Potsch-Schneider independently carried out GC subtyping of human semen stain with immobilized pH gradient gel isoelectric focusing (IPGIF) followed by ELISA in 1988. We report a new method there to subtype GC in human semen stains. First, the GC globulin was separated by polyacrylamide gel isoelectric focusing with pH 4.2-4.9 Pharmalyte. Then, it was immunoblotted to cellulose acetate membranes. Finally, the protein bands were shown by ELISA using horseradish peroxidase-linked secondary antibody system. All the 17 semen stains of volunteers were subtyped successfully. This method is easy to learn and convenient for applying in case work.
群体特异性成分（GC）是血清中一种具有高度遗传多态性的α2球蛋白。人类精液中也有少量的GC。1988年，普夫吕格和波奇-施奈德分别采用固定化pH梯度凝胶等电聚焦（IPGIF）结合酶联免疫吸附测定（ELISA）对人类精液斑进行GC分型。我们在此报告一种对人类精液斑中的GC进行分型的新方法。首先，使用pH 4.2 - 4.9 Pharmalyte通过聚丙烯酰胺凝胶等电聚焦分离GC球蛋白。然后，将其免疫印迹到醋酸纤维素膜上。最后，使用辣根过氧化物酶标记的二抗系统通过ELISA显示蛋白条带。所有17份志愿者的精液斑均成功分型。该方法易于学习，便于应用于实际案件工作。
