Amos L A, Jubb J S, Henderson R, Vigers G
J Mol Biol. 1984 Sep 25;178(3):711-29. doi: 10.1016/0022-2836(84)90248-1.
Tubulin crystals produced by incubation with vinblastine sulphate have been investigated by X-ray diffraction, electron microscopy and density measurement. The results show that crystals produced in vitro from bovine brain tubulin are made of packed helices, each consisting of a single continuous protofilament, whereas crystals produced in vivo in sea urchin eggs are built from pairs of such helices. The symmetry, cell dimensions, percentage protein and composition of the crystals are consistent with either 24 or 30 tubulin monomers per turn of the helix. The sea urchin egg crystals are more highly ordered and thus more suitable for further analysis.
通过硫酸长春碱孵育产生的微管蛋白晶体已通过X射线衍射、电子显微镜和密度测量进行了研究。结果表明，从牛脑微管蛋白体外产生的晶体由紧密堆积的螺旋组成，每个螺旋由单个连续的原纤维组成，而在海胆卵体内产生的晶体则由成对的这种螺旋构成。晶体的对称性、晶胞尺寸、蛋白质百分比和组成与每圈螺旋24或30个微管蛋白单体一致。海胆卵晶体的有序程度更高，因此更适合进一步分析。
