海胆卵子发生过程中蛋白质合成的研究。II. 微管蛋白的合成。

Studies of protein synthesis during sea urchin oogenesis. II. Synthesis of tubulin.

作者信息

Cognetti G, DI Liegro I, Cavarretta F

出版信息

Cell Differ. 1977 Aug;6(2):159-65. doi: 10.1016/0045-6039(77)90037-9.

Abstract

Sea urchin oocytes were incubated in vitro with [14C]lysine. Tubulin synthesis was demonstrated by gel filtration and/or electrophoresis of the colchicine-tubulin complex precipitated by vinblastine. The incorporation of [14C]lysine into tubulin represented about 12% of the incorporation into the total proteins at vitellogenesis.

摘要

将海胆卵母细胞与[14C]赖氨酸进行体外培养。通过对长春花碱沉淀的秋水仙碱-微管蛋白复合物进行凝胶过滤和/或电泳来证明微管蛋白的合成。在卵黄发生过程中，[14C]赖氨酸掺入微管蛋白的量约占掺入总蛋白量的12%。