Studies on simian hemorrhagic fever virus nucleocapsids.

Purified Simian Hemorrhagic Fever (SHF) virions are shown to have a sedimentation coefficient of 214S when compared with previously determined figures for Sindbis virus cosedimented in neutral sucrose gradients. The nucleic acid content in SHF virions was about 8 per cent of the total virionic mass. Nucleocapsids obtained by treating SHF virions with nonionic detergent and analyzed by ultracentrifugation in neutral sucrose gradients gave RNAse sensitive- but DNAse resistent particles with a reproducible sedimentation coefficient of 174S and a buoyant density of 1.33 g/ml in cesium chloride gradients.