Heine J, Moon H W, Woodmansee D B, Pohlenz J F
Vet Parasitol. 1984 Dec;17(1):17-25. doi: 10.1016/0304-4017(84)90061-x.
Tracheal and conjunctival infections with Cryptosporidium were established in pigs by inoculation of oocysts into the trachea and onto the conjunctival sacs. The protozoa were found attached to epithelial cells by an electron-dense band and a folded, vacuolated feeder organelle. They were situated in a parasitophorous vacuole surrounded by a double-layered membrane covered by glycocalyx. Trophozoites, schizonts, merozoites, macrogametes and oocysts could be demonstrated. In addition to normal stages of the life-cycle, degenerate stages were found. The infections with Cryptosporidium were characterized by focal destruction and loss of epithelial cells. Numerous intraepithelial lymphocytes were associated with affected foci, as were infiltrations with lymphocytes, monocytes, and macrophages.
通过将卵囊接种到猪的气管和结膜囊中，在猪身上建立了隐孢子虫引起的气管和结膜感染。通过电子致密带和折叠的、有空泡的摄食细胞器发现原生动物附着在上皮细胞上。它们位于被糖萼覆盖的双层膜包围的寄生泡中。可以观察到滋养体、裂殖体、裂殖子、大配子和卵囊。除了生命周期的正常阶段外，还发现了退化阶段。隐孢子虫感染的特征是上皮细胞的局灶性破坏和丧失。许多上皮内淋巴细胞与受影响的病灶相关，淋巴细胞、单核细胞和巨噬细胞的浸润也与之相关。
