Barnett S C, Eccles S A
Clin Exp Metastasis. 1984 Oct-Dec;2(4):297-310. doi: 10.1007/BF00135169.
A panel of fluorescein-conjugated lectins was used to investigate the cell surface carbohydrates of cell lines isolated from a mouse mammary adenocarcinoma which differ markedly in their morphological and metastatic properties. The lectin-binding profiles of the cells showed them to express generally similar cell surface characteristics; however, two minor differences were evident. Galactose moieties recognized by peanut lectin were expressed on all highly metastatic fusiform cell types examined, but only on 50-60 per cent of the polygonal cells of limited metastatic capacity. Similarly, N-acetylgalactosamine moieties were demonstrated on fusiform cell types by soya bean lectin binding but were not expressed on intact polygonal cells. In both cases pretreatment of polygonal cells with neuraminidase allowed lectin binding comparable with that of fusiform cells suggesting that Gal and GalNAc sugars were abundantly present but masked by sialic acid residues. Using a novel technique in which tumour cells were incubated on cryostat sections of normal tissues, it was found that the cell lines exhibited different adhesion patterns which to some extent reflected their preferential sites for spontaneous metastasis and organ colonization in vivo. Thus the adherence of fusiform cells to liver was five times as great as that of polygonal cells, whereas the latter bound preferentially to lung tissue. Prior treatment of polygonal cells with neuraminidase doubled their frequency of attachment to liver sections, but had no effect on their binding to other tissues. Also, the presence of 100 mM N-acetylgalactosamine during incubation specifically inhibited the adherence of fusiform cells to liver tissues, but did not significantly influence other cell-tissue interactions. The data suggest that the expression of galactosyl or N-acetylated galactosyl groups on the fusiform cells facilitates their attachment to lectin-like receptors on liver cells and contributes to their superior capacity, compared with polygonal cells, for growth and metastasis in this organ.
使用一组荧光素偶联凝集素来研究从小鼠乳腺腺癌分离出的细胞系的细胞表面碳水化合物，这些细胞系在形态和转移特性上有显著差异。细胞的凝集素结合谱显示它们通常具有相似的细胞表面特征；然而，有两个细微的差异很明显。花生凝集素识别的半乳糖部分在所有检测的高转移性梭形细胞类型上均有表达，但仅在50%-60%的转移能力有限的多边形细胞上表达。同样，大豆凝集素结合显示梭形细胞类型上有N-乙酰半乳糖胺部分，但完整的多边形细胞上不表达。在这两种情况下，用神经氨酸酶预处理多边形细胞可使凝集素结合与梭形细胞相当，这表明半乳糖（Gal）和N-乙酰半乳糖胺（GalNAc）糖大量存在，但被唾液酸残基掩盖。使用一种新技术，即将肿瘤细胞在正常组织的冰冻切片上孵育，发现这些细胞系表现出不同的黏附模式，这在一定程度上反映了它们在体内自发转移和器官定植的优先部位。因此，梭形细胞对肝脏的黏附力是多边形细胞的五倍，而后者优先结合肺组织。用神经氨酸酶预先处理多边形细胞可使其附着于肝脏切片的频率增加一倍，但对其与其他组织的结合没有影响。此外，孵育期间存在100 mM N-乙酰半乳糖胺可特异性抑制梭形细胞对肝脏组织的黏附，但对其他细胞-组织相互作用没有显著影响。数据表明，梭形细胞上半乳糖基或N-乙酰化半乳糖基的表达促进了它们与肝细胞上凝集素样受体的附着，并与多边形细胞相比，有助于它们在该器官中具有更强的生长和转移能力。
