兔肌肉醛缩酶A信使核糖核酸的完整核苷酸序列。
The complete nucleotide sequence for rabbit muscle aldolase A messenger RNA.
作者信息
Tolan D R, Amsden A B, Putney S D, Urdea M S, Penhoet E E
出版信息
J Biol Chem. 1984 Jan 25;259(2):1127-31.
The complete nucleotide sequence of rabbit muscle aldolase mRNA has been determined from recombinant cDNA clones and from a primer-extended cDNA synthesis on the mRNA template. The sequence is composed of 1375 nucleotides, exclusive of the poly(A) tails. The 5'-untranslated region contains 62 bases including a potential ribosome-binding site. The 3'-untranslated region is 221 bases long. The remaining 1092 nucleotides are in an open reading frame coding for 364 amino acids. There is a single methionine residue preceding the NH2-terminal proline. The deduced amino acid sequence corrects a number of discrepancies between published structures as well as assignments previously missed. The sequences of the cloned cDNAs suggests there may be microheterogeneity in the messenger RNA population.
已通过重组 cDNA 克隆以及在 mRNA 模板上进行引物延伸 cDNA 合成，确定了兔肌肉醛缩酶 mRNA 的完整核苷酸序列。该序列由 1375 个核苷酸组成，不包括 poly(A) 尾。5'-非翻译区包含 62 个碱基，包括一个潜在的核糖体结合位点。3'-非翻译区长 221 个碱基。其余 1092 个核苷酸位于编码 364 个氨基酸的开放阅读框中。在 NH2 端脯氨酸之前有一个甲硫氨酸残基。推导的氨基酸序列纠正了已发表结构之间的一些差异以及先前遗漏的归属。克隆的 cDNA 序列表明，信使 RNA 群体中可能存在微异质性。
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