Chowdhury N, DeRycke P H, Van Grembergen G
Z Parasitenkd. 1984;70(1):73-80. doi: 10.1007/BF00929576.
Hymenolepis microstoma has been cultured in vitro from 4 to 11 days of age using Eagle's medium (BME) supplemented with horse serum, lamb's liver extract and ox gall (dehydrated bile) in five different concentrations (0.025%, 0.1%, 0.2%, 0.5% and 1% all in BME) including a control without the added ox gall. The results of these experiments demonstrated that growth is related inversely to the concentration of ox gall in the media. However, the number of proglottids containing fertilized ova in the "lateral sacs" becomes higher in worms grown in the medium containing 0.025% ox bile than in those grown in the control medium. This number increases with the bile concentration up to 0.2%. It is concluded that bile stimulates oogenesis and in higher concentrations retards the growth of worms.
微小膜壳绦虫已在体外培养，培养对象为4至11日龄的虫体，使用的是添加了马血清、羊肝提取物和牛胆汁（脱水胆汁）的伊格尔培养基（基础培养基），牛胆汁有五种不同浓度（均在基础培养基中，分别为0.025%、0.1%、0.2%、0.5%和1%），包括一个未添加牛胆汁的对照。这些实验结果表明，生长与培养基中牛胆汁的浓度呈反比。然而，在含有0.025%牛胆汁的培养基中生长的虫体，其“侧囊”中含有受精卵的节片数量比在对照培养基中生长的虫体更高。这个数量随着胆汁浓度增加至0.2%而增多。得出的结论是，胆汁刺激卵子发生，而在较高浓度时会阻碍虫体生长。
