微小膜壳绦虫的体外研究。I.血红素化合物对生长和繁殖的影响。

Studies on Hymenolepis microstoma in vitro. I. Effect of heme compounds on growth and reproduction.

作者信息

Khan Z I, De Rycke P H

出版信息

Z Parasitenkd. 1976 Jun 10;49(3):253-61. doi: 10.1007/BF00380595.

Abstract

The effect of various heme compounds on growth and maturation of Hymenolepis microstoma in vitro has been determined. At 4 days p.i., worms were collected and cultured in vitro for 6 days in a medium containing various concentrations of haemoglobin, hemin, and bilirubin. Addition of haemoglobin to a medium resulted in significant increases in length, wet weight, number of immature and mature proglottids as compared to the control medium. Supplements of hemin did not seem to affect worm length; however, they caused a significant weight increase, and the numbers of immature and mature proglottids were also more than those in control medium. Bilirubin supplements failed to show any effect on growth and maturation of H. microstoma, and the experimental and control worms were found similar in all respects. In the light of the success with haemoglobin and hemin as growth promoting substances, the role of blood ingredients was also considered. For strobilization and maturation of H. microstoma in vitro, the presence of some kind of heme protein seems essential.

摘要

已确定各种血红素化合物对微小膜壳绦虫体外生长和成熟的影响。感染后4天，收集虫体并在含有不同浓度血红蛋白、血红素和胆红素的培养基中体外培养6天。与对照培养基相比，向培养基中添加血红蛋白会导致虫体长度、湿重、未成熟和成熟节片数量显著增加。添加血红素似乎不影响虫体长度；然而，它们会导致重量显著增加，未成熟和成熟节片的数量也比对照培养基中的多。添加胆红素对微小膜壳绦虫的生长和成熟没有任何影响，实验虫体和对照虫体在各方面都相似。鉴于血红蛋白和血红素作为生长促进物质取得的成功，还考虑了血液成分的作用。对于微小膜壳绦虫在体外的节裂和成熟，某种血红素蛋白的存在似乎至关重要。