Danielsen M, Stallcup M R
Mol Cell Biol. 1984 Mar;4(3):449-53. doi: 10.1128/mcb.4.3.449-453.1984.
The mouse thymoma-derived cell line W7 is sensitive to the cytolytic action of glucocorticoids. We have isolated a novel class of cell variant that apparently overcomes its inherent sensitivity to glucocorticoids by reversibly down-regulating the level of glucocorticoid receptors. This phenotype is stable during subcloning in the presence and in the absence of glucocorticoids and is dominant in somatic cell hybrids with wild-type cells. Fusion of this variant with wild-type cells produces hybrids that down-regulate and are less sensitive to glucocorticoids than hybrids of receptor-negative and wild-type cells. This is the first demonstration of a phenotypic change which correlates with down-regulation of the glucocorticoid receptor.
源自小鼠胸腺瘤的细胞系W7对糖皮质激素的细胞溶解作用敏感。我们分离出了一类新型细胞变体，该变体显然通过可逆性下调糖皮质激素受体水平克服了其对糖皮质激素的固有敏感性。在有或没有糖皮质激素存在的情况下进行亚克隆时，这种表型是稳定的，并且在与野生型细胞的体细胞杂交中占主导地位。该变体与野生型细胞融合产生的杂种细胞，相比于受体阴性和野生型细胞的杂种细胞，能下调糖皮质激素受体水平且对糖皮质激素的敏感性更低。这是首次证明与糖皮质激素受体下调相关的表型变化。
