Bensaude O, Morange M
Service de Génétique Cellulaire du College de France et de l'Institut Pasteur, Paris.
EMBO J. 1983;2(2):173-7. doi: 10.1002/j.1460-2075.1983.tb01401.x.
When submitted to a heat-shock, mouse embryonal carcinoma (EC) and fibroblast cells show very different behavior. All the EC cells so far analyzed express very high levels of several heat-shock proteins (HSP) in the absence of stress and independent of their origin and culture conditions. In such cells, the 89-kd, 70-kd and 59-kd HSP are the most prominent proteins after actin. In addition, the 89-kd and 59-kd HSP are not stimulated by an arsenite shock in contrast to what is observed with fibroblasts or cells of the parietal yolk sac type. Arsenite induces the synthesis of a 105-kd polypeptide in fibroblasts but not in EC cells. In vitro differentiation of F9 cells induced by retinoic acid and dibutyryl cAMP is accompanied by a decrease in the spontaneous relative abundance of HSP and restores the arsenite-induced synthesis of the 105-kd polypeptide. EC cells are usually believed to be similar to inner cell mass cells of mouse blastocyst. Furthermore, data in the literature together with our own results suggest that the same three HSP are also spontaneously expressed in high amounts in the early mouse embryo.
当受到热休克时，小鼠胚胎癌细胞（EC）和成纤维细胞表现出非常不同的行为。到目前为止分析的所有EC细胞在无应激状态下，无论其来源和培养条件如何，都表达非常高水平的几种热休克蛋白（HSP）。在这类细胞中，89-kd、70-kd和59-kd的HSP是继肌动蛋白之后最显著的蛋白质。此外，与成纤维细胞或壁层卵黄囊类型的细胞不同，89-kd和59-kd的HSP不会被亚砷酸盐休克所刺激。亚砷酸盐可诱导成纤维细胞合成一种105-kd的多肽，但不会在EC细胞中诱导合成。视黄酸和二丁酰环磷腺苷诱导的F9细胞体外分化伴随着HSP自发相对丰度的降低，并恢复了亚砷酸盐诱导的105-kd多肽的合成。EC细胞通常被认为与小鼠囊胚的内细胞团细胞相似。此外，文献中的数据以及我们自己的结果表明，相同的三种HSP在早期小鼠胚胎中也大量自发表达。
