胆酸钠与兔肺表面活性物质的相互作用。

Sodium cholate interactions with rabbit's pulmonary surfactant.

作者信息

Donoso P, Oyarzün M J, Segovia A, Guerrero M, Puig F

出版信息

Biol Neonate. 1984;45(5):252-6. doi: 10.1159/000242013.

DOI:10.1159/000242013

Abstract

In order to assay the possibility that sodium cholate interacts with pulmonary surfactant, we obtained bronchoalveolar lavage fluid from lungs of adult rabbits and measured the hysteresis area of surface tension-area loops of the bronchoalveolar lavage fluid in a Wilhelmy surface tension balance, before and after the addition of sodium cholate to reach different concentrations. We observed a biphasic behavior: at a low concentration of sodium cholate (1.5 x 10(5) mol/l; n = 6) the hysteresis area increased (p less than 0.05) as compared to its control (initial) area, meanwhile at a higher concentration (5 x 10(-5) mol/l; n = 6) the hysteresis area decreased (p less than 0.025), revealing a likely interaction of sodium cholate with pulmonary surfactant. We conclude that sodium cholate is able to interact in vitro with lung surfactant.

摘要

为了分析胆酸钠与肺表面活性剂相互作用的可能性，我们从成年兔的肺中获取支气管肺泡灌洗液，并在Wilhelmy表面张力天平上测量添加不同浓度胆酸钠前后支气管肺泡灌洗液表面张力-面积环的滞后面积。我们观察到一种双相行为：在低浓度胆酸钠（1.5×10⁻⁵ mol/l；n = 6）时，与对照（初始）面积相比，滞后面积增加（p < 0.05），而在较高浓度（5×10⁻⁵ mol/l；n = 6）时，滞后面积减小（p < 0.025），这表明胆酸钠可能与肺表面活性剂发生相互作用。我们得出结论，胆酸钠在体外能够与肺表面活性剂相互作用。