Vickers R M, Yu V L
J Clin Microbiol. 1984 May;19(5):583-7. doi: 10.1128/jcm.19.5.583-587.1984.
A systematic study of pigment production (browning) and fluorescence (extracellular yellow-green and intracellular blue-white) by nine Legionellaceae species was performed. A total of 56 strains representing Tatlockia micdadei (Pittsburgh pneumonia agent), Legionella pneumophila, Legionella jordanis, Legionella longbeachae, Legionella oakridgensis, Legionella wadsworthii, Fluoribacter bozemanae, Fluoribacter gormanii, and Fluoribacter dumoffii could be separated on media supplemented with tyrosine plus cystine, 3,4-diaminobenzoic acid, 3,5-diaminobenzoic acid, and 3-aminotyrosine. Parallel testing by hippurate hydrolysis and the bromocresol purple spot test enabled the identification of Legionellaceae species 24 to 72 h after primary isolation. This schema may be a practical alternative to species-specific antisera methods (slide agglutination or direct immunofluorescence) in the identification of members of the family Legionellaceae.
对9种军团菌科细菌产生色素（褐变）和荧光（细胞外黄绿色和细胞内蓝白色）的情况进行了系统研究。总共56株菌株，分别代表米克戴德嗜肺军团菌（匹兹堡肺炎病原体）、嗜肺军团菌、约旦军团菌、长滩军团菌、橡树岭军团菌、沃兹沃思军团菌、博兹曼荧光杆菌、戈尔曼荧光杆菌和杜莫夫荧光杆菌，可在添加酪氨酸加胱氨酸、3,4-二氨基苯甲酸、3,5-二氨基苯甲酸和3-氨基酪氨酸的培养基上分离。通过马尿酸盐水解和溴甲酚紫斑点试验进行平行检测，可在初次分离后24至72小时鉴定军团菌科细菌种类。在鉴定军团菌科成员时，该方法可能是物种特异性抗血清方法（玻片凝集或直接免疫荧光）的一种实用替代方法。
