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用于区分军团菌科成员的含染料缓冲活性炭酵母提取物培养基。

Dye-containing buffered charcoal-yeast extract medium for differentiation of members of the family Legionellaceae.

作者信息

Vickers R M, Brown A, Garrity G M

出版信息

J Clin Microbiol. 1981 Feb;13(2):380-2. doi: 10.1128/jcm.13.2.380-382.1981.

DOI:10.1128/jcm.13.2.380-382.1981
PMID:7009642
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC273792/
Abstract

The addition of 0.001% bromocresol purple and 0.001% bromothymol blue to buffered charcoal-yeast extract agar allowed differentiation between members of the family Legionellaceae. On this medium, Legionella pneumophila grew as relatively flat, pale green colonies, whereas Tatlockia micdadei (gen. nov., comb. nov., Pittsburgh pneumonia agent) produced blue-gray colonies. Fluoribacter spp. (gen. nov., atypical Legionella-like organisms) developed glistening colonies which were brighter green than those of L. pneumophila.

摘要

在缓冲活性炭酵母提取物琼脂中添加0.001%的溴甲酚紫和0.001%的溴百里酚蓝，可区分军团菌科的成员。在这种培养基上，嗜肺军团菌生长为相对扁平的淡绿色菌落，而米氏塔特洛克菌（新属，新组合，匹兹堡肺炎病原体）产生蓝灰色菌落。荧光杆菌属（新属，非典型军团菌样生物）形成闪亮的菌落，比嗜肺军团菌的菌落更亮绿。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3346/273792/d98053611033/jcm00163-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3346/273792/d98053611033/jcm00163-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3346/273792/d98053611033/jcm00163-0166-a.jpg

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本文引用的文献

1
Pittsburgh pneumonia agent: direct isolation from human lung tissue.匹兹堡肺炎病原体：直接从人肺组织中分离得到。
J Infect Dis. 1980 Jun;141(6):727-32. doi: 10.1093/infdis/141.6.727.
2
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J Clin Microbiol. 1979 Oct;10(4):437-41. doi: 10.1128/jcm.10.4.437-441.1979.
3
Atypical Legionella-like organisms: fastidious water-associated bacteria pathogenic for man.非典型军团菌样生物：对人类致病的苛求性水相关细菌。
水质作为建筑供水系统阳性结果的预测指标
Pathogens. 2019 Dec 13;8(4):295. doi: 10.3390/pathogens8040295.
4
The Legionella pneumophila Incomplete Phosphotransferase System Is Required for Optimal Intracellular Growth and Maximal Expression of PmrA-Regulated Effectors.嗜肺军团菌的不完全磷酸转移酶系统是细胞内最佳生长和PmrA调控效应子最大表达所必需的。
Infect Immun. 2017 May 23;85(6). doi: 10.1128/IAI.00121-17. Print 2017 Jun.
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Legionella and Legionnaires' disease: 25 years of investigation.军团菌与军团病：25年的研究历程
Clin Microbiol Rev. 2002 Jul;15(3):506-26. doi: 10.1128/CMR.15.3.506-526.2002.
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Multiplication of Legionella pneumophila in unsterilized tap water.嗜肺军团菌在未灭菌自来水中的繁殖。
Appl Environ Microbiol. 1982 Jun;43(6):1330-4. doi: 10.1128/aem.43.6.1330-1334.1982.
7
Hot water systems as sources of Legionella pneumophila in hospital and nonhospital plumbing fixtures.医院及非医院卫生管道装置中作为嗜肺军团菌来源的热水系统。
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Isolation of Pittsburgh pneumonia agent from a hospital shower.从医院淋浴喷头中分离出匹兹堡肺炎病原体。
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