Isolation and characterization of a novel disialoganglioside from bovine adrenal medulla.

A novel GDlb ganglioside which contains only N-glycolylneuraminic acid was isolated from bovine adrenal medulla by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The concentration of this ganglioside was 0.9 nmol lipid-bound sialic acid per gram fresh tissue, which accounted for 2.7% of the disialoganglioside fraction. The structure was elucidated by sugar analysis, neuraminidase digestion, and permethylation studies. The complete structure of this ganglioside was identified as GDlb (NeuGc)2 or II3 (NeuGc)2-GgOse4Cer.