牛肾上腺髓质中三唾液酸神经节苷脂的分离与鉴定

Isolation and characterization of the trisialogangliosides from bovine adrenal medulla.

作者信息

Ariga T, Sekine M, Yu R K, Miyatake T

出版信息

J Lipid Res. 1983 Jun;24(6):737-45.

Abstract

Trisialogangliosides were isolated from bovine adrenal medulla by DEAE-Sephadex A-25 and Iatrobeads column chromatography. Their structures were elucidated by sugar analysis, neuraminidase digestion, and permethylation studies. The complete structures of trisialogangliosides, A to D, were identified as follows. A: GT1b, IV3NeuAc, II3 (NeuAc)2-GgOse4Cer. B: GT1b(NeuAc/NeuAc-NeuGc-); IV3NeuAc, II3 (NeuAc alpha 2-8 NeuGc-)GgOse4Cer. C: GT1b (NeuGc/NeuAc-NeuAc-); IV3NeuGc, II3 (NeuAc alpha 2-8 NeuAc-)GgOse4Cer. D: GT1b (NeuAc/NeuGc-NeuGc-); IV3NeuAc, II3 (NeuGc alpha 2-8 NeuGc-)GgOse4Cer. Gangliosides B, C, and D, which contain N-glycolylneuraminic acid, have not previously been reported in the literature.

摘要

通过DEAE - 葡聚糖A - 25和离子交换树脂柱色谱法从牛肾上腺髓质中分离出三唾液酸神经节苷脂。通过糖分析、神经氨酸酶消化和全甲基化研究阐明了它们的结构。三唾液酸神经节苷脂A至D的完整结构鉴定如下。A：GT1b，IV3NeuAc，II3(NeuAc)2 - GgOse4Cer。B：GT1b(NeuAc/NeuAc - NeuGc - ); IV3NeuAc，II3(NeuAcα2 - 8 NeuGc - )GgOse4Cer。C：GT1b(NeuGc/NeuAc - NeuAc - ); IV3NeuGc，II3(NeuAcα2 - 8 NeuAc - )GgOse4Cer。D：GT1b(NeuAc/NeuGc - NeuGc - ); IV3NeuAc，II3(NeuGcα2 - 8 NeuGc - )GgOse4Cer。含有N - 羟乙酰神经氨酸的神经节苷脂B、C和D此前尚未见文献报道。