Tal B, Tamir I, Rokem J S, Goldberg I
Biochem J. 1984 Apr 15;219(2):619-24. doi: 10.1042/bj2190619.
The aglycon form of the steroidal sapogenin furost -5-ene-3 beta, 22,26-triol, 3 beta- chacotrioside 26 beta-D-glucopyranoside was isolated from cell suspension cultures of Dioscorea deltoidea and its molecular structure was determined by mass spectrometry and 1H and 13C n.m.r. spectroscopy. From kinetic studies and incorporation experiments with [1-14C]acetate it was concluded that the steroidal compound (in the glycoside form) is an intermediate in vivo in diosgenin biosynthesis. It accumulated in growing cells of D. deltoidea and was metabolized to diosgenin (in the glycoside form, i.e. dioscin ) in non-dividing cells.
从三角叶薯蓣的细胞悬浮培养物中分离出甾体皂苷元呋甾-5-烯-3β,22,26-三醇、3β-查考三糖苷26β-D-葡萄糖苷的苷元形式，并通过质谱法以及1H和13C核磁共振光谱法确定了其分子结构。通过动力学研究以及用[1-14C]乙酸盐进行的掺入实验得出结论，甾体化合物（糖苷形式）是薯蓣皂苷元生物合成过程中的体内中间体。它在三角叶薯蓣的生长细胞中积累，并在非分裂细胞中代谢为薯蓣皂苷元（糖苷形式，即薯蓣皂苷）。
