Sharp J K, Albersheim P, Ossowski P, Pilotti A, Garegg P, Lindberg B
J Biol Chem. 1984 Sep 25;259(18):11341-5.
The elicitor activity and structural characteristics of chemically synthesized hepta- and octa-beta-D-glucopyranosides were compared with the same properties of an elicitor-active mycelial-wall-derived hexa(beta-D-glucopyranosyl)-D-glucitol. The specific elicitor activities, retention times on reversed-phase liquid chromatography columns, glycosyl-linkage compositions, 1H NMR analyses, and glycosyl-sequence analyses of the synthetic and mycelial-wall-derived hexa(beta-D-glucopyranosyl)-D-glucitols were indistinguishable. This work provided conclusive proof that elicitor activity was associated with the structure proposed (Sharp, J. K., McNeil, M., and Albersheim, P. (1984) J. Biol. Chem. 259, 11321-11336) for the elicitor-active mycelial-wall-derived hexa(beta-D-glucopyranosyl)-D-glucitol.
将化学合成的七-β-D-吡喃葡萄糖苷和八-β-D-吡喃葡萄糖苷的诱导子活性及结构特征，与具有诱导子活性的菌丝壁衍生六（β-D-吡喃葡萄糖基）-D-葡萄糖醇的相同性质进行了比较。合成的和菌丝壁衍生的六（β-D-吡喃葡萄糖基）-D-葡萄糖醇的比诱导子活性、在反相液相色谱柱上的保留时间、糖基连接组成、1H NMR分析和糖基序列分析均无差异。这项工作提供了确凿证据，证明诱导子活性与为具有诱导子活性的菌丝壁衍生六（β-D-吡喃葡萄糖基）-D-葡萄糖醇所提出的结构（Sharp, J. K., McNeil, M., and Albersheim, P. (1984) J. Biol. Chem. 259, 11321-11336）相关联。
