Christofides N D, Polak J M, Bloom S R
Peptides. 1984 Mar-Apr;5(2):261-6. doi: 10.1016/0196-9781(84)90216-x.
We have developed a sensitive and specific radioimmunoassay to PHI and investigated its distribution in four mammalian species (man, cat, guinea-pig and rat). PHI was present in high concentrations, not only in intestine but also in brain, respiratory tract, urogenital tract and other peripheral tissues. Its distribution was similar to that of VIP and in each tissue examined there was always a significant correlation between the concentrations of these two peptides. In a survey of endocrine tumours, PHI was found to be produced only in those tumours that also produced VIP. In addition PHI was only elevated in the plasma of patients that also had high plasma VIP concentrations. This parallel distribution and release was found to be due to the co-synthesis of VIP and PHI in the same pro-hormone peptide. However, the variable ratio of VIP/PHI in different anatomical areas suggest that in these areas there is a different post-translational enzyme processing of the precursor protein.
我们已开发出一种针对胰高血糖素样肽I（PHI）的灵敏且特异的放射免疫测定法，并研究了其在四种哺乳动物（人、猫、豚鼠和大鼠）体内的分布情况。PHI不仅在肠道中浓度很高，在脑、呼吸道、泌尿生殖道及其他外周组织中也有高浓度存在。其分布与血管活性肠肽（VIP）相似，在所检测的每个组织中，这两种肽的浓度之间始终存在显著相关性。在内分泌肿瘤调查中，发现只有那些也产生VIP的肿瘤才会产生PHI。此外，只有血浆VIP浓度也高的患者血浆中PHI才会升高。发现这种平行分布和释放是由于VIP和PHI在同一前体激素肽中共合成。然而，不同解剖区域中VIP/PHI的可变比例表明，在这些区域中前体蛋白存在不同的翻译后酶加工过程。
