Amoscato A A, Babcock G F, Nishioka K
Peptides. 1984 May-Jun;5(3):489-94. doi: 10.1016/0196-9781(84)90075-5.
A 3,4-dehydroproline analogue of tuftsin (L-Thr-L-Lys-L-Pro-L-Arg) was prepared by the solid phase synthetic method. Following reversed-phase high performance liquid chromatography (HPLC) purification, the analogue was compared to tuftsin for its ability to to enhance the chemotactic, bactericidal and phagocytic activities of polymorphonuclear leukocytes (PMN). Both tuftsin and [delta 3-pro3]- tuftsin elicited a similar significant chemotactic effect at a concentration of 10 micrograms/ml. A slight suppression of the chemotactic activity was observed with tuftsin at 10(-3) micrograms/ml and with [delta 3-pro3]-tuftsin at concentrations of 10(-3), 10(-2) (significant) and 10-1 micrograms/ml. Although similar bactericidal activities were observed for both peptides, PMN exposed to [delta 3-pro 3]-tuftsin exhibited increased phagocytic indicies 2-4 times that of tuftsin-treated PMN at concentrations of 0.4, 0.6 and 1.0 microgram/ml.
通过固相合成法制备了促吞噬素（L-苏氨酸-L-赖氨酸-L-脯氨酸-L-精氨酸）的3,4-脱氢脯氨酸类似物。经反相高效液相色谱（HPLC）纯化后，将该类似物与促吞噬素在增强多形核白细胞（PMN）趋化、杀菌和吞噬活性方面的能力进行了比较。促吞噬素和[δ3-脯氨酸3]-促吞噬素在浓度为10微克/毫升时均引发了类似的显著趋化作用。在10^(-3)微克/毫升的促吞噬素以及浓度为10^(-3)、10^(-2)（显著）和10^(-1)微克/毫升的[δ3-脯氨酸3]-促吞噬素作用下，观察到趋化活性略有抑制。尽管两种肽表现出相似的杀菌活性，但在浓度为0.4、0.6和1.0微克/毫升时，暴露于[δ3-脯氨酸3]-促吞噬素的PMN的吞噬指数比经促吞噬素处理的PMN高2至4倍。
