[Monolayer culture of bone marrow and splenic fibroblasts in lymphoproliferative diseases].

The authors describe the results of monolayer cultivation bone marrow and spleen fibroblasts in patients with lymphoproliferative diseases. The efficacy of colony-formation of fibroblasts (ECFf) was studied in 10 patients with chronic lympholeukemia, 4 with leukemic reticuloendotheliosis, in 5 with lymphocytoma of the spleen, and in 3 with lymphosarcoma of the spleen. The control group comprised 7 normal subjects. Comparison of the ECFf on the spleen and bone marrow during lymphocytomas demonstrated the ECFf in the bone marrow to be low (0.24 +/- 0.16) in contrast to the high and normal ECFf in chronic lympholeukemia and leukemic reticuloendotheliosis. The ECFf in the spleen of patients with lymphocytomas was high (4.20 +/- 0.90), i. e. higher than in the control group (1.1 +/- 0.31). In lymphosarcoma of the spleen, the growth of fibroblasts was low in both the bone marrow and the spleen (0.15 +/- 0.13 and 0.10 +/- 0.01). These data suggest that the growth of stromal elements in lymphatic tumors is determined by the characteristic features of tumor cells.