人体胆囊化生病变的组织化学研究。

Histochemical studies of metaplastic lesions in the human gallbladder.

作者信息

Tsutsumi Y, Nagura H, Osamura Y, Watanabe K, Yanaihara N

出版信息

Arch Pathol Lab Med. 1984 Nov;108(11):917-21.

Abstract

We studied goblet cell metaplasia and pseudopyloric gland metaplasia in 25 surgically removed, paraffin-embedded gallbladder specimens using mucin histochemistry, silver methods for endocrine cells, and the indirect immunoperoxidase method for 12 peptide hormones, secretory component, and lysozyme. Goblet cell metaplasia was closely related to the occurrence of endocrine cells that showed argentaffinity, argyrophilia, or immunoreactive gastrin, somatostatin, pancreatic polypeptide, or motilin. Mucosal areas without goblet cell metaplasia were devoid of such endocrine cells. Metaplastic pseudopyloric glands showing lysozyme immunoreactivity were positive for class III mucin with paradoxical concanavalin A staining. Specimens with florid metaplastic lesions revealed a low tendency to form Rokitansky-Aschoff sinuses whose cells never showed a metaplastic nature. We compared the pathophysiological significance of metaplastic lesions in the gallbladder with intestinal metaplasia of the stomach.

摘要

我们使用黏液组织化学、内分泌细胞银染法以及针对12种肽类激素、分泌成分和溶菌酶的间接免疫过氧化物酶法，对25例手术切除并石蜡包埋的胆囊标本进行了杯状细胞化生和假幽门腺化生研究。杯状细胞化生与显示嗜银性、亲银性或免疫反应性胃泌素、生长抑素、胰多肽或胃动素的内分泌细胞的出现密切相关。无杯状细胞化生的黏膜区域不存在此类内分泌细胞。显示溶菌酶免疫反应性的化生假幽门腺对III类黏液呈阳性反应，伴刀豆球蛋白A染色异常。有明显化生病变的标本形成罗-阿窦的倾向较低，其细胞从未表现出化生性质。我们比较了胆囊化生病变与胃肠化生的病理生理意义。