Pace-Asciak C R, Micallef S
Can J Biochem Cell Biol. 1984 Aug;62(8):709-14. doi: 10.1139/o84-092.
6-Keto prostaglandin E1 (PGE1) was catabolized into two major products by homogenates of rat kidney in the presence of NAD. Rat brain was mostly deficient in this catabolic activity. A total profile of products from these experiments could be obtained using the pentafluorobenzyl esters, O-methyloxime, and trimethylsilyl either derivatives and analysis by capillary gas chromatography with electron capture detection. The same samples could be effectively analyzed by mass spectrometry using negative ion chemical ionization (NICI) detection. While 6-keto PGE1 showed spasmogenic activity on the rat stomach fundus as well as inhibition of the ADP-induced platelet aggregation, its catabolites lacked such activity. Analysis of rat whole blood by mass spectrometry using the NICI technique showed the presence of small amounts of 6-keto PGE1 and its 15-keto and 15-keto 13,14-dihydro catabolites in two of eight blood samples.
在烟酰胺腺嘌呤二核苷酸（NAD）存在的情况下，大鼠肾脏匀浆可将6-酮前列腺素E1（PGE1）分解为两种主要产物。大鼠大脑的这种分解代谢活性大多不足。使用五氟苄酯、O-甲基肟和三甲基硅烷基衍生物，并通过带有电子捕获检测的毛细管气相色谱法进行分析，可获得这些实验产物的完整图谱。使用负离子化学电离（NICI）检测的质谱法可有效分析相同的样品。虽然6-酮PGE1对大鼠胃底显示出致痉活性，并能抑制二磷酸腺苷（ADP）诱导的血小板聚集，但其分解代谢产物缺乏这种活性。使用NICI技术通过质谱法分析大鼠全血，结果显示在八个血液样本中的两个样本中存在少量的6-酮PGE1及其15-酮和15-酮-13,14-二氢分解代谢产物。
