不同泌乳阶段牛奶、婴儿配方奶粉和母乳中的维生素D、25-羟基维生素D和1,25-二羟基维生素D。

Vitamin D, 25-hydroxy-vitamin D and 1,25-dihydroxy-vitamin D in cow's milk, infant formulas and breast milk during different stages of lactation.

作者信息

Kunz C, Niesen M, von Lilienfeld-Toal H, Burmeister W

出版信息

Int J Vitam Nutr Res. 1984;54(2-3):141-8.

Abstract

Vitamin D and its metabolites were determined in cow's milk, infant formulas, and colostrum, transitional and mature breast milk by specific protein-binding assays following HPLC (high-pressure liquid chromatography). Cow's milk contains (mean +/- SEM) 50.4 +/- 4.1 pg/ml vitamin D (n = 10), 499 +/- 47 pg/ml 25-OH D (n = 10) and 9.7 +/- 1.0 pg/ml 1,25 (OH)2D (n = 3). 86.6% of the added vitamin D3 was recovered from infant formulas. The mean content (mean +/- SEM) of 25-OH D and 1,25(OH)2D of infant formulas are 299 +/- 35 pg/ml and 5.4 +/- 0.9 pg/ml (n = 9), respectively. Vitamin D concentration of colostrum and mature breast milk is 122 +/- 3.4 pg/ml (n = 7, mean +/- SEM) and 38 +/- 3.3 pg/ml (n = 9) respectively; the 25-OH D content increases from 294 +/- 50.6 pg/ml (n = 10) to 845 +/- 190 pg/ml (n = 14) during lactation. The increase of 1,25-(OH)2D from early to mature breast milk (3.2 +/- 0.6 pg/ml, n = 8 versus 5.3 +/- 0.7 pg/ml, n = 20) is still statistically significant.

摘要

采用高效液相色谱（HPLC）后特定蛋白质结合分析法测定了牛奶、婴儿配方奶粉、初乳、过渡乳和成熟母乳中的维生素D及其代谢产物。牛奶中维生素D含量（均值±标准误）为50.4±4.1 pg/ml（n = 10），25-羟基维生素D（25-OH D）含量为499±47 pg/ml（n = 10），1,25-二羟基维生素D（1,25(OH)2D）含量为9.7±1.0 pg/ml（n = 3）。婴儿配方奶粉中添加的维生素D3回收率为86.6%。婴儿配方奶粉中25-OH D和1,25(OH)2D的平均含量（均值±标准误）分别为299±35 pg/ml和5.4±0.9 pg/ml（n = 9）。初乳和成熟母乳中维生素D浓度分别为122±3.4 pg/ml（n = 7，均值±标准误）和38±3.3 pg/ml（n = 9）；哺乳期25-OH D含量从294±50.6 pg/ml（n = 10）增加到845±190 pg/ml（n = 14）。初乳到成熟母乳中1,25-(OH)2D的增加（早期母乳中为3.2±0.6 pg/ml，n = 8；成熟母乳中为5.3±0.7 pg/ml，n = 20）仍具有统计学意义。